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Literature summary for 3.1.3.26 extracted from

  • Greiner, R.; Konietzny, U.; Jany, K.D.
    Purification and properties of a phytase from rye (1998), J. Food Biochem., 22, 143-161.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.1-1 mM, slightly Secale cereale
Cu2+ 0.1-1 mM, strong Secale cereale
F-
-
Secale cereale
Hg2+ 0.1-1 mM, strong Secale cereale
Mn2+ 0.1-1 mM, slightly Secale cereale
molybdate
-
Secale cereale
phosphate
-
Secale cereale
vanadate
-
Secale cereale
wolframate
-
Secale cereale
Zn2+ 0.1-1 mM, strong Secale cereale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
myo-inositol hexakisphosphate
-
Secale cereale

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
1 * 66000, SDS-PAGE Secale cereale
67000
-
gel filtration Secale cereale

Organism

Organism UniProt Comment Textmining
Secale cereale
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Secale cereale

Source Tissue

Source Tissue Comment Organism Textmining
seed ungerminated Secale cereale
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O
-
Secale cereale 1-naphthol + phosphate
-
?
2-naphthyl phosphate + H2O
-
Secale cereale 2-naphthol + phosphate
-
?
ADP + H2O
-
Secale cereale ?
-
?
AMP + H2O
-
Secale cereale adenosine + phosphate
-
?
ATP + H2O
-
Secale cereale ?
-
?
D-fructose 6-phosphate + H2O
-
Secale cereale D-fructose + phosphate
-
?
diphosphate + H2O
-
Secale cereale 2 phosphate
-
?
fructose 1,6-diphosphate + H2O
-
Secale cereale ?
-
?
Glucose 6-phosphate + H2O
-
Secale cereale Glucose + phosphate
-
?
glycerol 2-phosphate + H2O
-
Secale cereale glycerol + phosphate
-
?
GTP + H2O
-
Secale cereale ?
-
?
myo-inositol hexakisphosphate + H2O
-
Secale cereale myo-inositol pentakisphosphate + phosphate
-
?
NADP+ + H2O
-
Secale cereale NAD+ + phosphate
-
?
O-phospho-L-Ser + H2O
-
Secale cereale Ser + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Secale cereale p-nitrophenol + phosphate
-
?
pyridoxal phosphate + H2O
-
Secale cereale pyridoxal + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE Secale cereale

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Secale cereale

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
90 min, no loss of activity up to Secale cereale
50
-
90 min, 15% loss of activity Secale cereale
60
-
90 min, 20% loss of activity Secale cereale

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
358
-
myo-inositol hexakisphosphate
-
Secale cereale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Secale cereale

pH Range

pH Minimum pH Maximum Comment Organism
3 6 inactive below pH 3.0 and above pH 6.0 Secale cereale

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5
-
4°C, 24 h, 70% loss of activity Secale cereale
3
-
4°C, rapid decline in activity Secale cereale
4
-
4°C, 10 days, less than 10% loss of activity Secale cereale
4 7.5 4°C, stable Secale cereale
8
-
4°C, 24 h, 46% loss of activity Secale cereale