Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.1-1 mM, slightly | Secale cereale | |
Cu2+ | 0.1-1 mM, strong | Secale cereale | |
F- | - |
Secale cereale | |
Hg2+ | 0.1-1 mM, strong | Secale cereale | |
Mn2+ | 0.1-1 mM, slightly | Secale cereale | |
molybdate | - |
Secale cereale | |
phosphate | - |
Secale cereale | |
vanadate | - |
Secale cereale | |
wolframate | - |
Secale cereale | |
Zn2+ | 0.1-1 mM, strong | Secale cereale |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
myo-inositol hexakisphosphate | - |
Secale cereale |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
1 * 66000, SDS-PAGE | Secale cereale |
67000 | - |
gel filtration | Secale cereale |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Secale cereale | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Secale cereale |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | ungerminated | Secale cereale | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | - |
Secale cereale | 1-naphthol + phosphate | - |
? | |
2-naphthyl phosphate + H2O | - |
Secale cereale | 2-naphthol + phosphate | - |
? | |
ADP + H2O | - |
Secale cereale | ? | - |
? | |
AMP + H2O | - |
Secale cereale | adenosine + phosphate | - |
? | |
ATP + H2O | - |
Secale cereale | ? | - |
? | |
D-fructose 6-phosphate + H2O | - |
Secale cereale | D-fructose + phosphate | - |
? | |
diphosphate + H2O | - |
Secale cereale | 2 phosphate | - |
? | |
fructose 1,6-diphosphate + H2O | - |
Secale cereale | ? | - |
? | |
Glucose 6-phosphate + H2O | - |
Secale cereale | Glucose + phosphate | - |
? | |
glycerol 2-phosphate + H2O | - |
Secale cereale | glycerol + phosphate | - |
? | |
GTP + H2O | - |
Secale cereale | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Secale cereale | myo-inositol pentakisphosphate + phosphate | - |
? | |
NADP+ + H2O | - |
Secale cereale | NAD+ + phosphate | - |
? | |
O-phospho-L-Ser + H2O | - |
Secale cereale | Ser + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Secale cereale | p-nitrophenol + phosphate | - |
? | |
pyridoxal phosphate + H2O | - |
Secale cereale | pyridoxal + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, SDS-PAGE | Secale cereale |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Secale cereale |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
90 min, no loss of activity up to | Secale cereale |
50 | - |
90 min, 15% loss of activity | Secale cereale |
60 | - |
90 min, 20% loss of activity | Secale cereale |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
358 | - |
myo-inositol hexakisphosphate | - |
Secale cereale |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Secale cereale |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | inactive below pH 3.0 and above pH 6.0 | Secale cereale |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
4°C, 24 h, 70% loss of activity | Secale cereale |
3 | - |
4°C, rapid decline in activity | Secale cereale |
4 | - |
4°C, 10 days, less than 10% loss of activity | Secale cereale |
4 | 7.5 | 4°C, stable | Secale cereale |
8 | - |
4°C, 24 h, 46% loss of activity | Secale cereale |