Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | the 45000 Da enzyme form shows maximal activity at 5 mM | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | 45000 Da enzyme form, at 1 mM | Saccharomyces cerevisiae | |
p-chloromercuriphenylsulfonic acid | 45000 Da enzyme form, at 1 mM | Saccharomyces cerevisiae | |
Phenylglyoxal | inhibition of the 45000 Da enzyme form and the 104000 Da enzyme form | Saccharomyces cerevisiae | |
propanolol | inhibition of the 45000 Da enzyme form and the 104000 Da enzyme form | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Saccharomyces cerevisiae | - |
- |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
plasma membrane | associated | Saccharomyces cerevisiae | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for the 45000 Da enzyme form, maximal activity at 1 mM | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | the 45000 Da enzyme form and 104000 Da enzyme form are induced when cells enter the stationary phase of growth | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
45000 Da enzyme form and 104000 Da enzyme form | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.35 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C, the 45000 Da enzyme form is stable for 3 months, the 104000 Da enzyme form is degraded to a 91000 Da enzyme form and retains full activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the 45000 Da enzyme form and 104000 Da enzyme form are induced when cells enter the stationary phase of growth | Saccharomyces cerevisiae | ? | - |
? | |
phosphatidic acid + H2O | - |
Saccharomyces cerevisiae | 1,2-diacyl-sn-glycerol + phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
45000 Da enzyme form, unstable above | Saccharomyces cerevisiae |
50 | - |
45000 Da enzyme form, complete inactivation after 20 min | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | 45000 Da enzyme form | Saccharomyces cerevisiae |