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Literature summary for 3.1.3.4 extracted from

  • Morlock, K.R.; McLaughlin, J.J.; Lin, Y.P.; Carman, G.M.
    Phosphatidate phosphatase from Saccharomyces cerevisiae. Isolation of 45- and 104-kDa forms of the enzyme that are differentially regulated by inositol (1991), J. Biol. Chem., 266, 3586-3593.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 the 45000 Da enzyme form shows maximal activity at 5 mM Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
NEM 45000 Da enzyme form, at 1 mM Saccharomyces cerevisiae
p-chloromercuriphenylsulfonic acid 45000 Da enzyme form, at 1 mM Saccharomyces cerevisiae
Phenylglyoxal inhibition of the 45000 Da enzyme form and the 104000 Da enzyme form Saccharomyces cerevisiae
propanolol inhibition of the 45000 Da enzyme form and the 104000 Da enzyme form Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Saccharomyces cerevisiae
-
-
mitochondrion
-
Saccharomyces cerevisiae 5739
-
plasma membrane associated Saccharomyces cerevisiae 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for the 45000 Da enzyme form, maximal activity at 1 mM Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae the 45000 Da enzyme form and 104000 Da enzyme form are induced when cells enter the stationary phase of growth ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
45000 Da enzyme form and 104000 Da enzyme form Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.35
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C, the 45000 Da enzyme form is stable for 3 months, the 104000 Da enzyme form is degraded to a 91000 Da enzyme form and retains full activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the 45000 Da enzyme form and 104000 Da enzyme form are induced when cells enter the stationary phase of growth Saccharomyces cerevisiae ?
-
?
phosphatidic acid + H2O
-
Saccharomyces cerevisiae 1,2-diacyl-sn-glycerol + phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
45000 Da enzyme form, unstable above Saccharomyces cerevisiae
50
-
45000 Da enzyme form, complete inactivation after 20 min Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 45000 Da enzyme form Saccharomyces cerevisiae