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Literature summary for 3.1.3.45 extracted from
- Purification and characterization of a specific 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase from Escherichia coli B (1980), J. Bacteriol., 142, 60-68.
General Stability
| General Stability | Organism |
|---|---|
| loss of activity upon repeated freezing and thawing | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Escherichia coli |  |
| Cd2+ | - |
Escherichia coli | |
| Cl- | - |
Escherichia coli | |
| Cu2+ | - |
Escherichia coli | |
| EDTA | - |
Escherichia coli | |
| Hg2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.058 | - |
3-deoxy-D-manno-octulosonate 8-phosphate | in presence of 1.0 mM Co2+, 37°C | Escherichia coli | |
| 0.091 | - |
3-deoxy-D-manno-octulosonate 8-phosphate | in presence of 1.0 mM Mg2+, 37°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 1.0 mM, stimulates to a lesser extent than Co2+ or Mg2+ | Escherichia coli | |
| Co2+ | 1.0 mM, optimal concentration for stimulation | Escherichia coli | |
| Mg2+ | 1.0 mM, optimal concentration for stimulation | Escherichia coli | |
| Mn2+ | 1.0 mM, stimulates to a lesser extent than Co2+ or Mg2+ | Escherichia coli | |
| Zn2+ | 1.0 mM, stimulates to a lesser extent than Co2+ or Mg2+ | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-deoxy-D-manno-octulosonate 8-phosphate + H2O | Escherichia coli | - |
3-deoxy-D-manno-octulosonate + phosphate | - |
? | |
| 3-deoxy-D-manno-octulosonate 8-phosphate + H2O | Escherichia coli B / ATCC 11303 | - |
3-deoxy-D-manno-octulosonate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.6 | - |
37°C | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -90°C, stable for up to 2 months in 0.02 M Tris-acetate buffer | Escherichia coli |
| 4°C, stable for at least 2 weeks | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-deoxy-D-manno-octulosonate 8-phosphate + H2O | - |
Escherichia coli | 3-deoxy-D-manno-octulosonate + phosphate | - |
? | |
| 3-deoxy-D-manno-octulosonate 8-phosphate + H2O | - |
Escherichia coli B / ATCC 11303 | 3-deoxy-D-manno-octulosonate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000-43000, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | - |
Escherichia coli |
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