Protein Variants | Comment | Organism |
---|---|---|
D181A | substrate-trapping mutant, kinetic and thermodynamic characterization | Homo sapiens |
D181A/Q262A | substrate-trapping mutant, kinetic and thermodynamic characterization, very high affinity for epidermal growth factor receptor | Homo sapiens |
additional information | design of further substrate-trapping mutants | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | - |
DADEpYLIPQQG | pH 7.0, 30°C, mutant Q262A | Homo sapiens | |
0.0026 | - |
DADEpYLIPQQG | pH 7.0, 25°C, wild type | Homo sapiens | |
0.051 | - |
DADEpYLIPQQG | pH 7.0, 30°C, mutant D181A/Q262A | Homo sapiens | |
0.056 | - |
DADEpYLIPQQG | pH 7.0, 25°C, mutant D181A | Homo sapiens | |
0.058 | - |
p-nitrophenyl phosphate | pH 7.0, 30°C, mutant D181A/Q262A | Homo sapiens | |
0.08 | - |
p-nitrophenyl phosphate | pH 7.0, 25°C, mutant D181A | Homo sapiens | |
2.4 | - |
p-nitrophenyl phosphate | pH 7.0, 25°C, wild type | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme PTP1B | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate | thermodynamic parameters | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DADEpYLIPQQG + H2O | - |
Homo sapiens | DADEYLIPQQG + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Homo sapiens | p-nitrophenol + phosphate | - |
? |