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Literature summary for 3.1.3.48 extracted from

  • Zhang, X.; Yang, S.; Li, X.; Zhu, P.; Xie, E.; Li, Z.
    Expression, purification, and characterization of a novel acid phosphatase that displays protein tyrosine phosphatases activity from Metarhizium anisopliae strain CQMa102 (2017), Biosci. Biotechnol. Biochem., 81, 2292-2300 .
    View publication on PubMed
Search for more literature for 3.1.3.48

Cloned(Commentary)

Cloned (Comment) Organism
gene ptpase, recombinant expression in Pichia pastoris Metarhizium anisopliae

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Metarhizium anisopliae
Cu2+
-
 Metarhizium anisopliae
dithiothreitol
-
 Metarhizium anisopliae
fluoride
-
 Metarhizium anisopliae
N-ethylmaleimide
-
 Metarhizium anisopliae
Zn2+
-
 Metarhizium anisopliae

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Metarhizium anisopliae
Ca2+ activates Metarhizium anisopliae
Co2+ activates Metarhizium anisopliae
Mg2+ activates Metarhizium anisopliae
Mn2+ activates Metarhizium anisopliae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[a protein]-tyrosine phosphate + H2O Metarhizium anisopliae
-
 [a protein]-tyrosine + phosphate
-
 ?
[a protein]-tyrosine phosphate + H2O Metarhizium anisopliae CQMa102
-
 [a protein]-tyrosine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Metarhizium anisopliae A1X864 i.e. Metarhizium acridum
-
Metarhizium anisopliae CQMa102 A1X864 i.e. Metarhizium acridum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity evaluation, the purified recombinant enzyme exhibits high activity on O-phospho-L-tyrosine Metarhizium anisopliae ?
-
 ?
additional information substrate specificity evaluation, the purified recombinant enzyme exhibits high activity on O-phospho-L-tyrosine Metarhizium anisopliae CQMa102 ?
-
 ?
O-phospho-L-tyrosine + H2O
-
 Metarhizium anisopliae tyrosine + phosphate
-
 ?
O-phospho-L-tyrosine + H2O
-
 Metarhizium anisopliae CQMa102 tyrosine + phosphate
-
 ?
[a protein]-tyrosine phosphate + H2O
-
 Metarhizium anisopliae [a protein]-tyrosine + phosphate
-
 ?
[a protein]-tyrosine phosphate + H2O
-
 Metarhizium anisopliae CQMa102 [a protein]-tyrosine + phosphate
-
 ?
[protein Golgi p230]-tyrosine phosphate + H2O
-
 Metarhizium anisopliae [protein Golgi p230]-tyrosine + phosphate
-
 ?
[protein Golgi p230]-tyrosine phosphate + H2O
-
 Metarhizium anisopliae CQMa102 [protein Golgi p230]-tyrosine + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 85000, about, recombinant enzyme Metarhizium anisopliae

Synonyms

Synonyms Comment Organism
acid phosphatase
-
 Metarhizium anisopliae
CQMa102 phosphatase
-
 Metarhizium anisopliae
protein tyrosine phosphatase
-
 Metarhizium anisopliae
PTPase
-
 Metarhizium anisopliae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 recombinant enzyme Metarhizium anisopliae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 recombinant enzyme Metarhizium anisopliae

pI Value

Organism Comment pI Value Maximum pI Value
Metarhizium anisopliae recombinant enzyme
-
 6.15

General Information

General Information Comment Organism
physiological function the protein tyrosine phosphatase (PTPase) plays an important role in insect immune system. The enzyme specifically dephosphorylate trans-Golgi p230 in vitro Metarhizium anisopliae