Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha,beta-methylene-adenosine 5'-diphosphate | inhibits isoforms: 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4 | Bos taurus | |
ATP | inhibits isoforms: 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4 | Bos taurus |
Application | Comment | Organism |
---|---|---|
medicine | target for a number of therapeutic drugs since increased levels of the enzyme correlate with various disease states | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
dCMP | isoenzyme 5'-NT-3 | Bos taurus | |
0.04 | - |
dCMP | isoenzyme 5'-NT-2 | Bos taurus | |
0.05 | - |
dCMP | isoenzyme 5'-NT-1 | Bos taurus | |
0.06 | - |
5'-AMP | isoenzyme 5'-NT-2 and 5'-NT-4 | Bos taurus | |
0.06 | - |
5'-IMP | isoenzyme 5'-NT-3 | Bos taurus | |
0.06 | - |
dCMP | isoenzyme 5'-NT-4 | Bos taurus | |
0.07 | - |
5'-IMP | isoenzyme 5'-NT-2 and 5'-NT-4 | Bos taurus | |
0.07 | - |
5'-GMP | isoenzyme 5'-NT-4 | Bos taurus | |
0.09 | - |
5'-GMP | isoenzyme 5'-NT-2 | Bos taurus | |
0.11 | - |
5'-AMP | isoenzyme 5'-NT-1 and 5'-NT-3 | Bos taurus | |
0.15 | - |
5'-IMP | isoenzyme 5'-NT-1 | Bos taurus | |
0.16 | - |
5'-GMP | isoenzyme 5'-NT-3 | Bos taurus | |
0.22 | - |
5'-GMP | isoenzyme 5'-NT-1 | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | glycosylphosphatidyl-inositol-anchored | Bos taurus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66200 | - |
x * 66200, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | differential glycosylation, especially in the oligosaccharides linked to Asn403, an increase in sialylated glycans, together with a decrease in high-mannose structures is observed arising from 5'-NT-1 to 5'-NT-4 | Bos taurus |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seminal plasma | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
300 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-AMP + H2O | - |
Bos taurus | adenosine + phosphate | - |
? | |
5'-GMP + H2O | - |
Bos taurus | guanosine + phosphate | - |
? | |
5'-IMP + H2O | - |
Bos taurus | inosine + phosphate | - |
? | |
dCMP + H2O | - |
Bos taurus | deoxycytidine + phosphate | - |
? | |
additional information | significant differences in substrate specificity between the isoforms are interpreted in terms of variable glycosylation causing steric hindrance of the substrate-binding site | Bos taurus | ? | - |
? | |
additional information | the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66200, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
5'-NT-1 | - |
Bos taurus |
5'-NT-2 | - |
Bos taurus |
5'-NT-3 | - |
Bos taurus |
5'-NT-4 | - |
Bos taurus |
ecto-5'-NT | - |
Bos taurus |
ecto-5'-nucleotidase | - |
Bos taurus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bos taurus | isoenzyme 5'-NT-4 | - |
6.1 |
Bos taurus | isoenzyme 5'-NT-3 | - |
6.2 |
Bos taurus | isoenzyme 5'-NT-2 | 6.8 | 6.3 |
Bos taurus | isoenzyme 5'-NT-1 | - |
6.5 |