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Literature summary for 3.1.3.5 extracted from
- Capillary electrophoresis-based nanoscale assays for monitoring ecto-5'-nucleotidase activity and inhibition in preparations of recombinant enzyme and melanoma cell membranes (2008), Anal. Biochem., 373, 129-140.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| catalytically active recombinant soluble glutathione-S transferase/ecto-5'-NT fusion protein is expressed in insect cells using the baculovirus system | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Homo sapiens |  |
| ADP | - |
Rattus norvegicus | |
| alpha,beta-methylene-ADP | - |
Homo sapiens | |
| alpha,beta-methylene-ADP | - |
Rattus norvegicus | |
| ATP | - |
Homo sapiens | |
| ATP | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
| membrane | - |
Rattus norvegicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent | Homo sapiens | |
| Mg2+ | dependent | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| agarose-coupled GSH affinity chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| melanoma cell | - |
Homo sapiens | - |
| melanoma cell | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-AMP + H2O | - |
Homo sapiens | adenosine + phosphate | - |
? | |
| 5'-AMP + H2O | 100% activity | Rattus norvegicus | adenosine + phosphate | - |
? | |
| 5'-CMP + H2O | 36% dephosphorylation in relation to AMP | Rattus norvegicus | cytidine + phosphate | - |
? | |
| 5'-GMP + H2O | 57% dephosphorylation in relation to AMP | Rattus norvegicus | guanosine + phosphate | - |
? | |
| 5'-UMP + H2O | 51% dephosphorylation in relation to AMP | Rattus norvegicus | uridine + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Homo sapiens | p-nitrophenol + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Rattus norvegicus | p-nitrophenol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CD73 | - |
Homo sapiens |
| CD73 | - |
Rattus norvegicus |
| ecto-5'-NT | - |
Homo sapiens |
| ecto-5'-NT | - |
Rattus norvegicus |
| ecto-5'-nucleotidase | - |
Homo sapiens |
| ecto-5'-nucleotidase | - |
Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00051 | - |
alpha,beta-methylene-ADP | in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 | Homo sapiens | |
| 0.00087 | - |
alpha,beta-methylene-ADP | in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 | Rattus norvegicus | |
| 0.00091 | - |
ADP | in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 | Rattus norvegicus | |
| 0.0089 | - |
ATP | in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 | Rattus norvegicus | |
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