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Literature summary for 3.1.3.58 extracted from

  • London, J.; Hausman, S.Z.; Thompson, J.
    Characterization of a membrane-regulated sugar phosphate phosphohydrolase from Lactobacillus casei (1985), J. Bacteriol., 163, 951-956.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.58

Inhibitors

Inhibitors Comment Organism Structure
Fe2+
-
 Lacticaseibacillus casei
Zn2+
-
 Lacticaseibacillus casei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane Lacticaseibacillus casei 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 5 mM, divalent cation required for maximal activity, doubles the rate of glucose 6-phosphate hydrolysis Lacticaseibacillus casei
Mg2+ 5 mM, divalent cation required for maximal activity, triples the rate of glucose 6-phosphate hydrolysis Lacticaseibacillus casei
additional information Mn2+ and Ni2+ have no effect on enzyme activity Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE Lacticaseibacillus casei
62000
-
 gel filtration Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + H2O Lacticaseibacillus casei key component of the futile xylitol cycle D-glucose + phosphate
-
 ?
D-xylitol-5-phosphate + H2O Lacticaseibacillus casei relative activity 34% D-xylitol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
 Cl-16
-
Lacticaseibacillus casei Cl-16
-
 Cl-16
-

Purification (Commentary)

Purification (Comment) Organism
partial Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.154
-
 2-deoxyglucose 6-phosphate as substrate Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Deoxy-D-glucose 6-phosphate + H2O relative activity 75% Lacticaseibacillus casei 2-Deoxy-D-glucose + phosphate
-
 ?
2-Deoxy-D-glucose 6-phosphate + H2O relative activity 75% Lacticaseibacillus casei Cl-16 2-Deoxy-D-glucose + phosphate
-
 ?
3-phosphoglycerate + H2O relative activity 4% Lacticaseibacillus casei glycerate + phosphate
-
 ?
3-phosphoglycerate + H2O relative activity 4% Lacticaseibacillus casei Cl-16 glycerate + phosphate
-
 ?
6-phosphogluconate + H2O relative activity 69% Lacticaseibacillus casei gluconate + phosphate
-
 ?
alpha-glycerophosphate + H2O relative activity 50% Lacticaseibacillus casei glycerol + phosphate
-
 ?
beta-D-glucose-1,6-diphosphate + H2O relative activity 56% Lacticaseibacillus casei beta-D-glucose + phosphate
-
 ?
D-erythrose-4-phosphate + H2O relative activity 69% Lacticaseibacillus casei D-erythrose + phosphate
-
 ?
D-fructose-1,6-diphosphate + H2O relative activity 16% Lacticaseibacillus casei D-fructose + phosphate
-
 ?
D-fructose-1-phosphate + H2O relative activity 8% Lacticaseibacillus casei D-fructose + phosphate
-
 ?
D-fructose-6-phosphate + H2O relative activity 47% Lacticaseibacillus casei D-fructose + phosphate
-
 ?
D-galactose-1-phosphate + H2O relative activity 8% Lacticaseibacillus casei D-galactose + phosphate
-
 ?
D-galactose-6-phosphate + H2O relative activity 11% Lacticaseibacillus casei D-galactose + phosphate
-
 ?
D-glucosamine-6-phosphate + H2O relative activity 28% Lacticaseibacillus casei D-glucosamine + phosphate
-
 ?
D-glucose 6-phosphate + H2O relative activity 100% Lacticaseibacillus casei D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O key component of the futile xylitol cycle Lacticaseibacillus casei D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O relative activity 100% Lacticaseibacillus casei Cl-16 D-glucose + phosphate
-
 ?
D-glucose-1-phosphate + H2O relative activity 3% Lacticaseibacillus casei D-glucose + phosphate
-
 ?
D-mannose 6-phosphate + H2O relative activity 80% Lacticaseibacillus casei D-mannose + phosphate
-
 ?
D-ribitol-5-phosphate + H2O relative activity 15% Lacticaseibacillus casei D-ribitol + phosphate
-
 ?
D-ribose 5-phosphate + H2O relative activity 53% Lacticaseibacillus casei D-ribose + phosphate
-
 ?
D-ribose 5-phosphate + H2O relative activity 53% Lacticaseibacillus casei Cl-16 D-ribose + phosphate
-
 ?
D-sedoheptulose-7-phosphate + H2O relative activity 34% Lacticaseibacillus casei sedoheptulose + phosphate
-
 ?
D-sorbitol-6-phosphate + H2O relative activity 33% Lacticaseibacillus casei D-sorbitol + phosphate
-
 ?
D-xylitol-5-phosphate + H2O relative activity 34% Lacticaseibacillus casei D-xylitol + phosphate
-
 ?
D-xylose-1-phosphate + H2O relative activity 3% Lacticaseibacillus casei D-xylose + phosphate
-
 ?
mannitol-1-phosphate + H2O relative activity 16% Lacticaseibacillus casei D-mannitol + phosphate
-
 ?
additional information little or no affinity for substrates phosphorylated at the C-1 position, acts on sugars and polyols phosphorylated on the terminal carbon, with a preference for sugars with a D-erythro-configuration, phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane, no activity detected with mannose-1-phosphate, maltose-1-phosphate, 2-phosphoglycerate, ribose-1-phosphate, phosphoenolpyruvate, beta-glycerophosphate, phosphothreonine, phosphoserine, and para-nitrophenyl phosphate Lacticaseibacillus casei ?
-
 ?
additional information little or no affinity for substrates phosphorylated at the C-1 position, acts on sugars and polyols phosphorylated on the terminal carbon, with a preference for sugars with a D-erythro-configuration, phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane, no activity detected with mannose-1-phosphate, maltose-1-phosphate, 2-phosphoglycerate, ribose-1-phosphate, phosphoenolpyruvate, beta-glycerophosphate, phosphothreonine, phosphoserine, and para-nitrophenyl phosphate Lacticaseibacillus casei Cl-16 ?
-
 ?
o-nitrophenyl D-galactose-6-phosphate + H2O relative activity 6% Lacticaseibacillus casei o-nitrophenyl D-galactose + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Lacticaseibacillus casei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Lacticaseibacillus casei