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Literature summary for 3.1.3.59 extracted from
- 1-O-alkyl-2-acetyl-sn-glycero-3-phosphate:phosphohydrolase activity in Tetrahymena pyriformis (2000), J. Eukaryot. Microbiol., 47, 122-128.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Tween 20 | 20% increase of activity | Tetrahymena pyriformis |  |
| Tween 80 | 20% increase of activity | Tetrahymena pyriformis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-chloro-2,3-dinitrobenzene | pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition | Tetrahymena pyriformis | |
| acyl-glycerolphosphate | 38% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction | Tetrahymena pyriformis | |
| alkyl-glycerolphosphate | 58% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction | Tetrahymena pyriformis | |
| Ca2+ | - |
Tetrahymena pyriformis | |
| dithiothreitol | - |
Tetrahymena pyriformis | |
| Mg2+ | - |
Tetrahymena pyriformis | |
| additional information | no inhibition: diacyl-glycerolphosphate, D-fructose-6-phosphate, 2-glycerophosphate, ATP, 1-alkyl-2-acetyl-sn-glycerol | Tetrahymena pyriformis | |
| N-ethylmaleimide | pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition | Tetrahymena pyriformis | |
| Nonidet | 100% inhibition | Tetrahymena pyriformis | |
| SDS | 100% inhibition | Tetrahymena pyriformis | |
| sodium deoxycholate | 100% inhibition | Tetrahymena pyriformis | |
| Triton-X100 | 0.2% causes 100% inhibition | Tetrahymena pyriformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
1-Alkyl-2-acetyl-sn-glycero-3-phosphate | pH 7.0, 37°C, mitochondrial and microsomal fractions | Tetrahymena pyriformis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | 15% of the total activity | Tetrahymena pyriformis | 5829 | - |
| membrane | - |
Tetrahymena pyriformis | 16020 | - |
| microsome | 20% of the total activity | Tetrahymena pyriformis | - |
- |
| mitochondrion | 40% of the total activity | Tetrahymena pyriformis | 5739 | - |
| pellicle | 20% of the total activity | Tetrahymena pyriformis | 20039 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Tetrahymena pyriformis | enzyme may be involved in the de novo production of platelet-activating factor biosynthesis | ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | EDTA and EGTA no affect on enzyme activity | Tetrahymena pyriformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetrahymena pyriformis | - |
protozoan | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Tetrahymena pyriformis |
| 0.0031 | - |
cytosol | Tetrahymena pyriformis |
| 0.0159 | - |
pellicle | Tetrahymena pyriformis |
| 0.0164 | - |
mitochondrion and microsome | Tetrahymena pyriformis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stable for 3 months | Tetrahymena pyriformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-acyl-2-acetyl-sn-glycero-3-phosphate + H2O | - |
Tetrahymena pyriformis | 1-acyl-2-acetyl-sn-glycerol + phosphate | - |
? | |
| 1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O | best substrate | Tetrahymena pyriformis | 1-alkyl-2-acetyl-sn-glycerol + phosphate | - |
? | |
| 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O | - |
Tetrahymena pyriformis | ? | - |
? | |
| alkyl-glycerophosphate + H2O | - |
Tetrahymena pyriformis | alkylglycerol + phosphate | - |
? | |
| diacyl-glycerophosphate + H2O | - |
Tetrahymena pyriformis | dialkylglycerol + phosphate | poor substrate | ? | |
| additional information | enzyme may be involved in the de novo production of platelet-activating factor biosynthesis | Tetrahymena pyriformis | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | stable | Tetrahymena pyriformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
microsome and mitochondrion | Tetrahymena pyriformis |
| 9 | - |
microsome | Tetrahymena pyriformis |
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