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Literature summary for 3.1.3.8 extracted from

  • Jareonkitmongkol, S.; Ohya, M.; Watanabe, R.; Takagi, H.; Nakamori, S.
    Partial purification of phytase from a soil isolate bacterium Klebsiella oxytoca MO-3 (1997), J. Ferment. Bioeng., 83, 393-394.
No PubMed abstract available
Search for more literature for 3.1.3.8

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Klebsiella oxytoca
EDTA
-
 Klebsiella oxytoca
F- NaF Klebsiella oxytoca
Fe2+
-
 Klebsiella oxytoca
NEM
-
 Klebsiella oxytoca
Zn2+
-
 Klebsiella oxytoca

Organism

Organism UniProt Comment Textmining
Klebsiella oxytoca
-
-
-
Klebsiella oxytoca
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
Klebsiella oxytoca MO-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Klebsiella oxytoca

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45.3
-
-
 Klebsiella oxytoca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca 4-nitrophenol + phosphate
-
 ?
AMP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca adenosine + phosphate
-
 ?
AMP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 adenosine + phosphate
-
 ?
ATP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca ?
-
 ?
ATP + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 ?
-
 ?
diphosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca 2 phosphate
-
 ?
diphosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 2 phosphate
-
 ?
Glucose 6-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca Glucose + phosphate
-
 ?
Glucose 6-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 Glucose + phosphate
-
 ?
glycerol 2-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca glycerol + phosphate
-
 ?
glycerol 2-phosphate + H2O less than 10% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca MO-3 glycerol + phosphate
-
 ?
inositol tetraphosphate + H2O almost the same activity as with myo-inositol hexakisphosphate Klebsiella oxytoca ?
-
 ?
inositol triphosphate + H2O 40% of the activity with myo-inositol hexakisphosphate Klebsiella oxytoca ?
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Klebsiella oxytoca 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 20 min, stable Klebsiella oxytoca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Klebsiella oxytoca

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 stable Klebsiella oxytoca