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Literature summary for 3.1.3.8 extracted from
- Phytase activity in Cryptococcus laurentii ABO 510 (2007), FEMS Yeast Res., 7, 442-448.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | may be considered for application as an animal feed additive to assist in the hydrolysis of phytate complexes to improve the bioavailability of phosphorus in plant feedstuff | Papiliotrema laurentii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 77.12% residual activity at 5 mM | Papiliotrema laurentii |  |
| Ba2+ | 71.95% residual activity at 5 mM | Papiliotrema laurentii | |
| Ca2+ | 68.64% residual activity at 5 mM | Papiliotrema laurentii | |
| Cd2+ | 40.54% residual activity at 1 mM | Papiliotrema laurentii | |
| Co2+ | 76.08% residual activity at 5 mM | Papiliotrema laurentii | |
| Cu2+ | 14.03% residual activity at 5 mM | Papiliotrema laurentii | |
| Fe2+ | 41.3% residual activity at 5 mM | Papiliotrema laurentii | |
| Hg2+ | 31.17% residual activity at 5 mM | Papiliotrema laurentii | |
| KH2PO4 | complete inhibition at 0.5 mM | Papiliotrema laurentii | |
| Mn2+ | 36.01% residual activity at 5 mM | Papiliotrema laurentii | |
| myo-inositol hexakisphosphate | completely inhibited by 5 mM myo-inositol hexakisphosphate | Papiliotrema laurentii | |
| Ni(2+) | 79.25% residual activity at 5 mM | Papiliotrema laurentii | |
| phosphate | completely inhibited by 0.5 mM phosphate | Papiliotrema laurentii | |
| Zn2+ | 27,34% residual activity at 5 mM | Papiliotrema laurentii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
myo-inositol hexakisphosphate | at 62°C, in 50 mM sodium acetate buffer (pH 5) | Papiliotrema laurentii | |
| 0.07 | - |
ATP | at 62°C, in 50 mM sodium acetate buffer (pH 5) | Papiliotrema laurentii | |
| 0.14 | - |
D-fructose 1,6-diphosphate | at 62°C, in 50 mM sodium acetate buffer (pH 5) | Papiliotrema laurentii | |
| 0.17 | - |
di-sodium phenyl phosphate dihydrate | at 62°C, in 50 mM sodium acetate buffer (pH 5) | Papiliotrema laurentii | |
| 0.27 | - |
D-glucose 6-phosphate | at 62°C, in 50 mM sodium acetate buffer (pH 5) | Papiliotrema laurentii | |
| 0.28 | - |
D-glucose 1-phosphate | at 62°C, in 50 mM sodium acetate buffer (pH 5) | Papiliotrema laurentii | |
| 0.52 | - |
p-nitrophenyl phosphate | at 62°C, in 50 mM sodium acetate buffer (pH 5) | Papiliotrema laurentii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Li+, Na+, K+ and Mg2+ do not significantly affect the phytase activity | Papiliotrema laurentii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Papiliotrema laurentii | - |
- |
- |
| Papiliotrema laurentii ABO 510 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | 76.67% activity compared to myo-inositol hexakisphosphate | Papiliotrema laurentii | ADP + phosphate | - |
? | |
| ATP + H2O | 76.67% activity compared to myo-inositol hexakisphosphate | Papiliotrema laurentii ABO 510 | ADP + phosphate | - |
? | |
| D-fructose 1,6-diphosphate + H2O | 202.19% activity compared to myo-inositol hexakisphosphate | Papiliotrema laurentii | ? | - |
? | |
| D-fructose 1,6-diphosphate + H2O | 202.19% activity compared to myo-inositol hexakisphosphate | Papiliotrema laurentii ABO 510 | ? | - |
? | |
| D-glucose 1-phosphate + H2O | 76.72% activity compared to myo-inositol hexakisphosphate | Papiliotrema laurentii | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | 73.75% activity compared to myo-inositol hexakisphosphate | Papiliotrema laurentii | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | 73.75% activity compared to myo-inositol hexakisphosphate | Papiliotrema laurentii ABO 510 | D-glucose + phosphate | - |
? | |
| di-sodium phenyl phosphate dihydrate + H2O | 111.85% activity compared to myo-inositol hexakisphosphate | Papiliotrema laurentii | ? | - |
? | |
| myo-inositol hexakisphosphate + H2O | preferred substrate, 100% activity | Papiliotrema laurentii | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | preferred substrate, 100% activity | Papiliotrema laurentii ABO 510 | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | 152.95% activity compared to myo-inositol hexakisphosphate | Papiliotrema laurentii | p-nitrophenol + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | 152.95% activity compared to myo-inositol hexakisphosphate | Papiliotrema laurentii ABO 510 | p-nitrophenol + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 62 | - |
- |
Papiliotrema laurentii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | 80 | - |
Papiliotrema laurentii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 70 | thermostable between 4°C and 70°C | Papiliotrema laurentii |
| 70 | 90 | approximately 40% of the original activity is lost after exposure to 70°C for 180 min, with no residual activity being seen after exposure to 80°C for 60 min or 90°C for 5 min | Papiliotrema laurentii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Papiliotrema laurentii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 7.5 | - |
Papiliotrema laurentii |
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