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Literature summary for 3.1.30.1 extracted from

  • Harada, F.; Dahlberg, J.E.
    Specific cleavage of tRNA by nuclease S1 (1975), Nucleic Acids Res., 2, 865-871.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis analysis of tRNA for determination of amino acid specificity Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Aspergillus oryzae
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
RNA + H2O Aspergillus oryzae
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
RNA + H2O
-
Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
tRNA + H2O in the anticodon region Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Synonyms

Synonyms Comment Organism
S1 nuclease
-
Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 with tRNA as substrate Aspergillus oryzae