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Literature summary for 3.1.30.1 extracted from
- Endonucleases specific for single-stranded polynucleotides (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 193-201.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | - |
Vigna radiata var. radiata |
| analysis | - |
Neurospora crassa |
| analysis | in DNA-RNA annaeling experiments as reagent for selective removal of nonhybridized polynucleotides | Aspergillus oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-ATP | 50% inhibition by 0.5 mM | Neurospora crassa |  |
| EDTA | - |
Aspergillus oryzae | |
| EDTA | 95% inhibition at 0.1 mM, reversed by equivalent amounts of Co2+ | Neurospora crassa | |
| EDTA | no activity after dialysis with 1 mM, no restoration by addition of Zn2+, Co2+, Mg2+, Mn2+, Ca2+ or cysteine | Vigna radiata var. radiata | |
| NaCl | optimal activity at 100 mM, 55% of maximal rate at 400 mM | Aspergillus oryzae | |
| phosphate | - |
Aspergillus oryzae | |
| phosphate | 80% inhibition by 33 mM potassium phosphate, pH 7.5 | Neurospora crassa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Neurospora crassa | |
| Co2+ | - |
Aspergillus oryzae | |
| Zn2+ | - |
Vigna radiata var. radiata | |
| Zn2+ | - |
Neurospora crassa | |
| Zn2+ | - |
Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
- |
Aspergillus oryzae |
| 39000 | - |
reduction with 2-mercaptoethanol prior to SDS-PAGE generates two polypeptides of 25000 and 15000, linked covalently by disulfide bond, intact and cleaved forms are equally active | Vigna radiata var. radiata |
| 42000 | - |
gel filtration | Vigna radiata var. radiata |
| 43000 | - |
sucrose density gradient sedimentation | Vigna radiata var. radiata |
| 55000 | - |
- |
Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Vigna radiata var. radiata | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| DNA + H2O | Aspergillus oryzae | functional role in recombination and DNA repair in vivo | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| DNA + H2O | Neurospora crassa | native DNA hydrolysis probably by contaminating nuclease, its inactivation possible at 55°C | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| RNA + H2O | Vigna radiata var. radiata | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| RNA + H2O | Neurospora crassa | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| RNA + H2O | Aspergillus oryzae | 5fold more activity on DNA than RNA | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
| Neurospora crassa | - |
- |
- |
| Vigna radiata var. radiata | - |
mung bean | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus oryzae |
| glycoprotein | 29% carbohydrate by weight | Vigna radiata var. radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| conidium | - |
Neurospora crassa | - |
| mycelium | stationary phase | Neurospora crassa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| DNA + H2O | functional role in recombination and DNA repair in vivo | Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| DNA + H2O | native DNA hydrolysis probably by contaminating nuclease, its inactivation possible at 55°C | Neurospora crassa | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| RNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| RNA + H2O | - |
Neurospora crassa | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| RNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| RNA + H2O | - |
Neurospora crassa | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | mainly 5'-phosphodi- and trinucleotides, at long incubation time more than 90% 5'-phosphomononucleotides | ? | |
| RNA + H2O | 5fold more activity on DNA than RNA | Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| ssDNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| ssDNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| ssDNA + H2O | - |
Neurospora crassa | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | mainly 5'-phosphodi- and trinucleotides, at long incubation time more than 90% 5'-phosphomononucleotides | ? | |
| tRNA + H2O | - |
Neurospora crassa | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| tRNA + H2O | hydrolysis of nonhydrogen-bonded loops | Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mung bean endonuclease | - |
Vigna radiata var. radiata |
| S1 nuclease | - |
Aspergillus oryzae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | - |
Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 4.3 | - |
Aspergillus oryzae |
| 5 | - |
because of rapid inactivation, stabilization by 0.1 mM Zn2+ or 1 mM cysteine, glutathione or dithiothreitol recommended | Vigna radiata var. radiata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Neurospora crassa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
5'-ATP | - |
Neurospora crassa | |
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