Literature summary for 3.1.30.1 extracted from

Martin, S.A.; Ullrich, R.C.; Meyer, W.L.
A comparative study of nucleases exhibiting preference for single-stranded nucleic acid (1986), Biochim. Biophys. Acta, 867, 76-80.

No PubMed abstract available

Search for more literature for 3.1.30.1

Application

Application	Comment	Organism
analysis	-	Vigna radiata var. radiata
analysis	-	Neurospora crassa
analysis	-	Penicillium citrinum
analysis	studies of nucleic acid secondary structure	Aspergillus oryzae
analysis	studies of nucleic acid secondary structure	Schizophyllum commune

Inhibitors

Inhibitors	Comment	Organism	Structure
8-hydroxychinoline 5-sulfonate	10% of maximal activity with 0.01 mM, no activity with 0.1 mM	Neurospora crassa
8-hydroxychinoline 5-sulfonate	-	Pisum sativum
8-hydroxychinoline 5-sulfonate	8% of maximal activity with 0.01 mM, no activity with 0.1 mM	Schizophyllum commune
8-hydroxychinoline 5-sulfonate	30% of maximal activity with 0.01 mM	Vigna radiata var. radiata
EDTA	-	Aspergillus oryzae
EDTA	almost complete inactivation by 0.01 mM	Neurospora crassa
EDTA	-	Pisum sativum
EDTA	15% of maximal activity with 0.01 mM	Schizophyllum commune
EDTA	almost complete inactivation by 0.01 mM	Vigna radiata var. radiata
F-	complete inhibition by 10 mM	Neurospora crassa
F-	18% of maximal activity with 10 mM	Schizophyllum commune
F-	5% of maximal activity with 10 mM	Vigna radiata var. radiata

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	-	Neurospora crassa
Co2+	-	Aspergillus oryzae
Co2+	-	Ustilago maydis
Co2+	-	Penicillium citrinum
Co2+	-	Schizophyllum commune
Zn2+	-	Vigna radiata var. radiata
Zn2+	-	Triticum aestivum
Zn2+	-	Neurospora crassa
Zn2+	-	Physarum polycephalum
Zn2+	-	Aspergillus oryzae
Zn2+	-	Ustilago maydis
Zn2+	-	Penicillium citrinum
Zn2+	-	Schizophyllum commune

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	-	Aspergillus oryzae
32000	-	gel filtration	Physarum polycephalum
34000	-	gel filtration	Schizophyllum commune
34000	35000	gel filtration	Vigna radiata var. radiata
34000	35000	gel filtration	Aspergillus oryzae
34000	35000	gel filtration	Schizophyllum commune
39000	-	-	Vigna radiata var. radiata
43000	-	-	Vigna radiata var. radiata
43000	-	gel filtration	Triticum aestivum
44000	-	gel filtration	Penicillium citrinum
47000	-	gel filtration	Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA + H2O	Vigna radiata var. radiata	-	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	Triticum aestivum	-	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	Neurospora crassa	-	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	Pisum sativum	-	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	Physarum polycephalum	-	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	Aspergillus oryzae	-	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	Ustilago maydis	-	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	Penicillium citrinum	-	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	Schizophyllum commune	-	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus oryzae	-	-	-
Neurospora crassa	-	-	-
Penicillium citrinum	-	-	-
Physarum polycephalum	-	-	-
Pisum sativum	-	-	-
Schizophyllum commune	-	-	-
Triticum aestivum	-	-	-
Ustilago maydis	-	-	-
Vigna radiata var. radiata	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	-	Vigna radiata var. radiata	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	-	Triticum aestivum	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	-	Neurospora crassa	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	-	Pisum sativum	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	-	Physarum polycephalum	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	-	Aspergillus oryzae	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	-	Ustilago maydis	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	-	Penicillium citrinum	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
DNA + H2O	-	Schizophyllum commune	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
ssDNA + H2O	-	Vigna radiata var. radiata	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
ssDNA + H2O	-	Triticum aestivum	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
ssDNA + H2O	-	Neurospora crassa	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
ssDNA + H2O	-	Pisum sativum	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
ssDNA + H2O	-	Physarum polycephalum	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
ssDNA + H2O	-	Aspergillus oryzae	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
ssDNA + H2O	-	Ustilago maydis	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
ssDNA + H2O	-	Penicillium citrinum	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
ssDNA + H2O	-	Schizophyllum commune	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?

Synonyms

Synonyms	Comment	Organism
mung bean nuclease I	-	Vigna radiata var. radiata
Neurospora crassa endonuclease	-	Neurospora crassa
S1 nuclease	-	Aspergillus oryzae
SC nuclease	-	Schizophyllum commune
Ustilago maydis nuclease	-	Ustilago maydis

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Release 2023.1 (January 2023)