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Literature summary for 3.1.30.1 extracted from

  • Reddy, L.G.; Shankar, V.
    Immobilization of single-strand specific nuclease (S1 nuclease) from Aspergillus oryzae (1987), Appl. Biochem. Biotechnol., 14, 231-240.
    View publication on PubMed
Search for more literature for 3.1.30.1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Aspergillus oryzae
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
ssDNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

Synonyms Comment Organism
S1 nuclease
-
 Aspergillus oryzae
single-strand-specific nuclease
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Aspergillus oryzae
55
-
 soluble enzyme Aspergillus oryzae
65
-
 immobilized enzyme Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 at 70°C complete loss of activity of the soluble enzyme, 40% of maximal activity of the immobilized enzyme at this temperature, 20% at 80°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8 4.3 pH 3.8 for the immobilized, pH 4.3 for the soluble enzyme Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.6 5.2 comparatively higher stability of the immobilized than the soluble enzyme Aspergillus oryzae