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Literature summary for 3.1.30.1 extracted from
- S1 nuclease of Aspergillus oryzae: a glycoprotein with an associated nucleatidase activity (1980), Arch. Biochem. Biophys., 204, 361-370.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | studies of nucleic acid secondary structure | Aspergillus oryzae |
| analysis | reagent for mapping splice points and ordered regions in RNA | Aspergillus oryzae |
| synthesis | industrial production of 5'-phosphomononucleotides from yeast RNA | Aspergillus oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | with 1 mM stabilization of activity at pH 3.5 and 4.0 | Aspergillus oryzae |  |
| NaCl | maximal activity at 0-100 mM, at 1 mM 71% of maximal activity | Aspergillus oryzae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
3'-AMP | - |
Aspergillus oryzae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 46% stimulation of activity at 0.1 mM Zn2+, maximal stimulation at 0.01 mM | Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
SDS-PAGE | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Aspergillus oryzae | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus oryzae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus oryzae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.1 M buffer pH 3.5-4.0, mercaptoethanol, 22 h, 16.9% of initial activity at pH 3.5, 59% at pH 4.0, by preincubation with Zn2+ 48% of initial activity at pH 3.5, 86% at pH 4.0 | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-AMP + H2O | - |
Aspergillus oryzae | adenosine + phosphate | - |
? | |
| 3'-AMP + H2O | - |
Aspergillus oryzae | adenosine + phosphate | - |
? | |
| 3'-CMP + H2O | - |
Aspergillus oryzae | cytosine + phosphate | - |
? | |
| 3'-dAMP + H2O | - |
Aspergillus oryzae | 3'-deoxyadenosine + phosphate | - |
? | |
| 3'-dTMP + H2O | - |
Aspergillus oryzae | deoxythymidine + phosphate | - |
? | |
| 3'-GMP + H2O | - |
Aspergillus oryzae | guanosine + phosphate | - |
? | |
| 3'-UMP + H2O | - |
Aspergillus oryzae | uridine + phosphate | - |
? | |
| adenosine 3',5'-bisphosphate + H2O | - |
Aspergillus oryzae | 5'-AMP + phosphate | - |
? | |
| DNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| ssDNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S1 nuclease | - |
Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | with 3'-AMP and adenosine 3',5'-biphosphate as substrate | Aspergillus oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8.5 | with mononucleotide substrates | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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