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Literature summary for 3.1.30.1 extracted from
- Characterization of S1 nuclease. Involvement of carboxylate groups in metal binding (1992), Eur. J. Biochem., 210, 437-441.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| carboxylate group modification | at pH 4.6, 50-85% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 5-15 mM 1-ethyl-3-(dimethylaminopropyl)-carbodiimide; at pH 7.8, 55-95% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 2-15 mM Woodward's reagent K | Aspergillus oryzae | |
| EDTA | 55-100% loss of initial activity with 1-10 mM | Aspergillus oryzae |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | with 1 mM restoration of 72% of activity of 1 mM EDTA-treated enzyme | Aspergillus oryzae | |
| Zn2+ | with 1 mM restoration of 76% of activity of 1 mM EDTA-treated enzyme | Aspergillus oryzae | |
| Zn2+ | metalloprotein with one loosely and two strongly bound zinc atoms | Aspergillus oryzae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Aspergillus oryzae | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| RNA + H2O | Aspergillus oryzae | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus oryzae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme after dialysis against deionized water, no loss of activity | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-AMP + H2O | - |
Aspergillus oryzae | adenosine + phosphate | - |
? | |
| DNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| RNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| ssDNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S1 nuclease | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
- |
Aspergillus oryzae |
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Release 2023.1 (January 2023)
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