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Literature summary for 3.1.30.1 extracted from
- Single-strand-specific nuclease from the nucleoplasm of rye germ nuclei (1980), Eur. J. Biochem., 108, 285-293.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | strong inhibition above 100 mM | Secale cereale |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleoplasm | - |
Secale cereale | 5654 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
SDS-PAGE | Secale cereale |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Secale cereale | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Secale cereale | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from nucleoplasm | Secale cereale |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-AMP + H2O | - |
Secale cereale | adenosine + phosphate | - |
? | |
| DNA + H2O | - |
Secale cereale | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| dsDNA + H2O | cleavage of phage PM2 superhelical DNA to relaxed, nicked-circular form and unit length linear form molecules | Secale cereale | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| polyadenylic acid + H2O | - |
Secale cereale | 5'-AMP + 5'-phosphooligonucleotides | - |
? | |
| polycytosylic acid + H2O | only small degradation | Secale cereale | 5'-CMP + 5'-phosphooligonucleotides | - |
? | |
| polyguanylic acid + H2O | only small degradation | Secale cereale | 5'-GMP + 5'-phosphooligonucleotides | - |
? | |
| polyuridylic acid + H2O | - |
Secale cereale | 5'-UMP + 5'-phosphooligonucleotides | - |
? | |
| ssDNA + H2O | - |
Secale cereale | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| rye germ nuclease | - |
Secale cereale |
| single-strand-specific nuclease | - |
Secale cereale |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
- |
Secale cereale |
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Release 2023.1 (January 2023)
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