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Literature summary for 3.1.30.1 extracted from

  • Ikeda, S.; Maeda, N.; Ohshima, T.; Takata, N.
    Identification and characterization of a mitochondrial endonuclease from yeast, Schizosaccharomyces pombe (1996), Biochem. Mol. Biol. Int., 40, 1017-1024.
    View publication on PubMed
Search for more literature for 3.1.30.1

Inhibitors

Inhibitors Comment Organism Structure
aurintricarboxylic acid complete inhibition at 0.005 mM Schizosaccharomyces pombe
Ca2+ about 10% of activity towards ssDNA at 1 and 10 mM Schizosaccharomyces pombe
Ionic strength about 40% of activity towards ssDNA at 0.2 M NaCl and KCl Schizosaccharomyces pombe
Mg2+ reduction of activity towards ssDNA at 10 mM Schizosaccharomyces pombe
Zn2+ about 15% of activity towards ssDNA at 1 and 10 mM Schizosaccharomyces pombe

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Schizosaccharomyces pombe 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Schizosaccharomyces pombe
Mn2+ required for activity, reduced activity towards ssDNA at 10 mM Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 SDS-PAGE Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Schizosaccharomyces pombe
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
RNA + H2O Schizosaccharomyces pombe
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Schizosaccharomyces pombe 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
dsDNA + H2O
-
 Schizosaccharomyces pombe 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
RNA + H2O
-
 Schizosaccharomyces pombe 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
ssDNA + H2O
-
 Schizosaccharomyces pombe 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

Synonyms Comment Organism
Schizosaccharomyces pombe endonuclease
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Schizosaccharomyces pombe