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Literature summary for 3.1.30.1 extracted from
- Single-strand-specific nuclease of pea seeds: glycoprotein nature and associated nucleotidase activity (1987), Arch. Biochem. Biophys., 255, 437-445.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | almost complete inhibition of 3'-AMP hydrolysis by 1 mM CoCl2 | Pisum sativum |  |
| EDTA | almost complete inhibition of 3'-AMP hydrolysis with 1 mM | Pisum sativum | |
| Hg2+ | complete inhibition of 3'-AMP hydrolysis by 1 mM HgCl2 | Pisum sativum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1.7fold stimulation of 3'-AMP hydrolysis with 5 mM CaCl2 | Pisum sativum | |
| Mg2+ | 2fold stimulation of 3'-AMP hydrolysis with 5 mM MgCl2 | Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
alpha,beta, 1 * 30200 + 1 * 24000, SDS-PAGE | Pisum sativum |
| 30200 | - |
alpha,beta, 1 * 30200 + 1 * 24000, SDS-PAGE | Pisum sativum |
| 54000 | - |
native enzyme | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Pisum sativum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | about 20% carbohydrate | Pisum sativum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | germinating | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
purified enzyme, DNA as substrate | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Pisum sativum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| dsDNA + H2O | higher rate of hydrolysis of alkylated and depurinated dsDNA than native DNA | Pisum sativum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| ssDNA + H2O | - |
Pisum sativum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha,beta, 1 * 30200 + 1 * 24000, SDS-PAGE | Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
with native DNA or 3'-AMP as substrate | Pisum sativum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
after 90 min incubation time 90% of activity towards 3'-AMP and 60% of activity towards DNA lost | Pisum sativum |
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Release 2023.1 (January 2023)
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