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Literature summary for 3.1.30.2 extracted from

  • Romanova, J.; Filimonova, M.
    The effects of addition of mononucleotides on Sma nuc endonuclease activity (2012), ScientificWorldJournal, 2012, 454176.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.30.2

Inhibitors

Inhibitors Comment Organism Structure
AMP competitive inhibitor of partial type, AMP forming a complex with Sma nuc does not completely prevent the RNA binding and reduces the enzyme affinity to RNA substrate in competition with RNA for the binding site Serratia marcescens
CMP uncompetitive inhibitor Serratia marcescens
dAMP competitive inhibitor of partial type, dAMP forming a complex with Sma nuc does not completely prevent the RNA binding and reduces the enzyme affinity to RNA substrate in competition with RNA for the binding site Serratia marcescens
GMP uncompetitive inhibitor Serratia marcescens
UMP uncompetitive inhibitor Serratia marcescens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens P13717 isoform Sm2 of Sma nuc endonuclease
-

Synonyms

Synonyms Comment Organism
Sma nuc endonuclease
-
 Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Serratia marcescens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics of mononucleotides, hyperbolic inhibition of the enzyme, overview Serratia marcescens
0.06
-
 dAMP pH 8.5, 37°C Serratia marcescens
0.0765
-
 AMP pH 8.5, 37°C Serratia marcescens
0.0862
-
 CMP pH 8.5, 37°C Serratia marcescens
0.229
-
 GMP pH 8.5, 37°C Serratia marcescens
0.3107
-
 UMP pH 8.5, 37°C Serratia marcescens

General Information

General Information Comment Organism
additional information specific site(s) for the nucleotide(s) binding in Sma nuc endonuclease Serratia marcescens
physiological function any mononucleotide produced by Sma nuc during hydrolysis of DNA or RNA may regulate the enzyme activity affecting the RNase activity without pronounced influence on the activity towards DNA. The type of carbohydrate residue in mononucleotides does not affect the regulation. In contrast, the effects depend on the type of bases in nucleotides Serratia marcescens