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Literature summary for 3.1.4.3 extracted from

  • Pawelczyk, T.; Matecki, A.
    Expression, purification and kinetic properties of human recombinant phospholipase C delta 3 (1997), Acta Biochim. Pol., 44, 221-229.
    View publication on PubMed
Search for more literature for 3.1.4.3

Activating Compound

Activating Compound Comment Organism Structure
histone PLC d1 and PLC d3 Homo sapiens
lysozyme PLC d1 Homo sapiens
Melittin PLC d1 and PLC d3 Homo sapiens
putrescine PLC d1 Homo sapiens
spermidine PLC d1 Homo sapiens
spermine PLC d1 and PLC d3 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
tranformed in Escherichia coli Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ recombinant PLC d3: maximum activity with 0.001 mM, recombinant PLC d1: maximum activity with 0.01 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylinositol-bisphosphate + H2O Homo sapiens
-
 inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PLC, cation exchange chromatography, 26fold to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53.13
-
 substrate: phosphatidylinositol-4,5-bisphosphate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylinositol-bisphosphate + H2O
-
 Homo sapiens inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
 ?