Literature summary for 3.1.4.35 extracted from

Kuwayama, H.; Snippe, H.; Derks, M.; Roelofs, J.; Van Haastert, P.J.
Identification and characterization of DdPDE3, a cGMP-selective phosphodiesterase from Dictyostelium (2001), Biochem. J., 353, 635-644.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	not activated by 8-bromo-cGMP	Dictyostelium discoideum

Cloned(Commentary)

Cloned (Comment)	Organism
expression of DdPDE3 catalytic domain in Escherichia coli and Dictyostelium	Dictyostelium discoideum

Inhibitors

Inhibitors	Comment	Organism	Structure
3-isobutyl-1-methylxanthine	0.06 mM, 50% inhibition	Dictyostelium discoideum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00022	-	cGMP	-	Dictyostelium discoideum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity, maximal activity at 3-10 mM MgCl2	Dictyostelium discoideum
Mn2+	approx. 2.5fold higher activation than with Mg2+, maximal activity at 3-10 mM Mn2+	Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
guanosine 3',5'-cyclic phosphate + H2O	Dictyostelium discoideum	very low activity with cAMP	guanosine 5'-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Dictyostelium discoideum	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
guanosine 3',5'-cyclic phosphate + H2O	-	Dictyostelium discoideum	guanosine 5'-phosphate	-	?
guanosine 3',5'-cyclic phosphate + H2O	very low activity with cAMP	Dictyostelium discoideum	guanosine 5'-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
cGMP-selective phosphodiesterase	-	Dictyostelium discoideum
DdPDE3	-	Dictyostelium discoideum

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Release 2023.1 (January 2023)