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Literature summary for 3.1.4.37 extracted from

  • Lee, J.; Gravel, M.; Gao, E.; O'Neill, R.C.; Braun, P.E.
    Identification of essential residues in 2',3'-cyclic nucleotide 3'-phosphodiesterase. Chemical modification and site-directed mutagenesis to investigate the role of cysteine and histidine residues in enzymatic activity (2001), J. Biol. Chem., 276, 14804-14813.
    View publication on PubMed
Search for more literature for 3.1.4.37

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant CNP1, expression in Escherichia coli BL21 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C231A CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
C231S CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
C236A CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
C236S CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
C314A CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
C314S CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
C397S CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
H230F almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 Rattus norvegicus
H230L almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 Rattus norvegicus
H309F almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 Rattus norvegicus
H309L almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 Rattus norvegicus
additional information CNP1: N-terminal deletion mutants, catalytic fragment corresponding to the last C-terminal 250 amino acids Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2'-AMP product inhibitor Rattus norvegicus
5,5'-dithiobis-(2-nitrobenzoic acid) 97% inhibition of the catalytic fragment of CNP1 in a time- and dose-dependent manner, kinetics, fully reversed by excess dithiothreitol or 2-mercaptoethanol, inhibition is attributable to steric effects of modification of Cys-236 and Cys-314 by the inhibitor Rattus norvegicus
diethylpyrocarbonate pH 6.5, 22°C, time-dependent inhibition, kinetics, completely reversed by 0.5 M hydroxylamine Rattus norvegicus
KCN 62% inhibition of the catalytic fragment of CNP1 Rattus norvegicus
methyl methanethiosulfonate 42% inhibition of the catalytic fragment of CNP1 Rattus norvegicus
additional information not inhibited by dithiothreitol Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.098
-
 2',3'-cNADP pH 6, 25°C, H309L mutant CNP1 Rattus norvegicus
0.1
-
 2',3'-cNADP pH 6, 25°C, H309F mutant CNP1 Rattus norvegicus
0.104
-
 2',3'-cNADP pH 6, 25°C, H230L mutant CNP1 Rattus norvegicus
0.119
-
 2',3'-cNADP pH 6, 25°C, H230F mutant CNP1 Rattus norvegicus
0.231
-
 2',3'-cNADP pH 6, 25°C, C231A mutant CNP1 Rattus norvegicus
0.237
-
 2',3'-cNADP pH 6, 25°C, recombinant full-length CNP1 Rattus norvegicus
0.241
-
 2',3'-cNADP pH 6, 25°C, C314S mutant CNP1 Rattus norvegicus
0.295
-
 2',3'-cNADP pH 6, 25°C, catalytic fragment of CNP1 corresponding to the C-terminal 250 amino acids Rattus norvegicus
0.333
-
 2',3'-cNADP pH 6, 25°C, C314A mutant CNP1 Rattus norvegicus
0.354
-
 2',3'-cNADP pH 6, 25°C, C236A mutant CNP1 Rattus norvegicus
0.379
-
 2',3'-cNADP pH 6, 25°C, C236S mutant CNP1 Rattus norvegicus
0.473
-
 2',3'-cNADP pH 6, 25°C, C231S mutant CNP1 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 x * 46000, CNP1, x * 48000, CNP2 Rattus norvegicus
48000
-
 x * 46000, CNP1, x * 48000, CNP2 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus CNP is one of the earliest myelin-related proteins to be expressed in differentiated oligodendrocytes and Schwann cells, role in migration and/or expansion of membranes during myelination ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol at a final concentration up to 4% v/v of the total volume, no effect on CNP1 activity Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 2 isoforms: CNP1 and CNP2 differing by a 20-amino acid extension at the N-terminus
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification isoprenylated at Cys-397 Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant CNP1 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cNADP+ + H2O
-
 Rattus norvegicus NADP+
-
 ?
2',3'-cyclic NADP+ + H2O
-
 Rattus norvegicus NADP+
-
 ?
additional information CNP is one of the earliest myelin-related proteins to be expressed in differentiated oligodendrocytes and Schwann cells, role in migration and/or expansion of membranes during myelination Rattus norvegicus ?
-
 ?
nucleoside 2',3'-cyclic phosphate + H2O His-230 and His-309 of CNP1 play critical roles in enzyme catalysis, no essential role of cysteines, the catalytic domain forms a compact globular structure Rattus norvegicus nucleoside 2'-phosphate
-
 ir

Subunits

Subunits Comment Organism
? x * 46000, CNP1, x * 48000, CNP2 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.15
-
 2',3'-cNADP pH 6, 25°C, H230F mutant CNP1 Rattus norvegicus
1.16
-
 2',3'-cNADP pH 6, 25°C, H230L mutant CNP1 Rattus norvegicus
1.27
-
 2',3'-cNADP pH 6, 25°C, H309L mutant CNP1 Rattus norvegicus
1.35
-
 2',3'-cNADP pH 6, 25°C, H309F mutant CNP1 Rattus norvegicus
6.08
-
 2',3'-cNADP pH 6, 25°C, H230L mutant CNP1 Rattus norvegicus
6.08
-
 2',3'-cNADP pH 6, 25°C, H309L mutant CNP1 Rattus norvegicus
594
-
 2',3'-cNADP pH 6, 25°C, C314S mutant CNP1 Rattus norvegicus
825
-
 2',3'-cNADP pH 6, 25°C, C231S mutant CNP1 Rattus norvegicus
968
-
 2',3'-cNADP pH 6, 25°C, C231A mutant CNP1 Rattus norvegicus
1107
-
 2',3'-cNADP pH 6, 25°C, C236S mutant CNP1 Rattus norvegicus
1110
-
 2',3'-cNADP pH 6, 25°C, C236S mutant CNP1 Rattus norvegicus
1116
-
 2',3'-cNADP pH 6, 25°C, C314A mutant CNP1 Rattus norvegicus
1120
-
 2',3'-cNADP pH 6, 25°C, C314A mutant CNP1 Rattus norvegicus
1130
-
 2',3'-cNADP pH 6, 25°C, C397S mutant CNP1 Rattus norvegicus
1132
-
 2',3'-cNADP pH 6, 25°C, C397S mutant CNP1 Rattus norvegicus
1195
-
 2',3'-cNADP pH 6, 25°C, recombinant full-length wild-type CNP1 Rattus norvegicus
1200
-
 2',3'-cNADP pH 6, 25°C, recombinant full-length wild-type CNP1 Rattus norvegicus
1476
-
 2',3'-cNADP pH 6, 25°C, C236A mutant CNP1 Rattus norvegicus
1480
-
 2',3'-cNADP pH 6, 25°C, C236A mutant CNP1 Rattus norvegicus
1690
-
 2',3'-cNADP pH 6, 25°C, catalytic fragment of CNP1 corresponding to the C-terminal 250 amino acids Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
 2'-AMP
-
 Rattus norvegicus