Activating Compound | Comment | Organism | Structure |
---|---|---|---|
putrescine | activity is increased 6fold at 10 mM | Rattus norvegicus | |
spermidine | EC50: about 1 mM | Rattus norvegicus | |
spermine | activation is observed only in the enzyme preparations after solubilization. EC50: about 0.1 mM. Synergistic activation of the enzyme by spermine and CaCl2 or by spermine and Triton X-100 is not observed | Rattus norvegicus | |
Triton X-100 | maximally active at 0.05%, higher concentrations reduce activity. Synergistic activation of the enzyme by spermine and CaCl2 or by spermine and Triton X-100 is not observed | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
N-palmitoyl-1,2-dioleoyl-phosphatidylethanolamine | pH 7.5, 37°C, in presence of 1 mM spermine | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation is observed only in the enzyme preparations after solubilization, EC50: 3 mM. Synergistic activation of the enzyme by spermine and CaCl2 or by spermine and Triton X-100 is not observed | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q769K2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-arachidonoyl-1,2-dioleoyl-phosphatidylehanolamine + H2O | hydrolysis rate with 0.1 mM N-arachidonoyl-1,2-dioleoyl-phosphatidylehanolamine is about 50% of that with 0.1 mM N-palmitoyl-1,2-dioleoyl-phosphatidylethanolamine | Rattus norvegicus | N-arachidonoylethanolamine + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
N-palmitoyl-1,2-dioleoyl-phosphatidylethanolamine + H2O | - |
Rattus norvegicus | N-palmitoylethanolamine + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |