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Literature summary for 3.1.6.1 extracted from

  • Amoscato, A.A.; Brumfield, A.M.; Sansoni, S.B.; Herberman, R.B.; Chambers, W.H.
    Natural killer cell cytolytic granule-associated enzymes. I. Purification, characterization, and analyis of function of an enzyme with sulfatase activity (1991), J. Immunol., 147, 950-958.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
PO43-
-
Rattus norvegicus
SDS
-
Rattus norvegicus
SO32-
-
Rattus norvegicus
SO42-
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
4-nitrocatechol sulfate
-
Rattus norvegicus
26
-
4-nitrophenyl sulfate
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
specific granule
-
Rattus norvegicus 42581
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
the enzyme has the ability to complex to molecular masses of more than 300000, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
leukemia cell line
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.23
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrocatechol sulfate + H2O
-
Rattus norvegicus 4-nitrocatechol + sulfate
-
?
4-nitrophenyl sulfate + H2O
-
Rattus norvegicus 4-nitrophenol + sulfate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
5 h, 30% loss of activity Rattus norvegicus
56
-
5 h, 51% loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 4-nitrophenyl sulfate Rattus norvegicus
5.6
-
hydrolysis of 3-nitrocatechol sulfate Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: about 55% of maximal activity, pH 7.0: about 35% of maximal activity, hydrolysis of 4-nitrophenyl sulfate Rattus norvegicus
5 7 pH 5.0: about 75% of maximal activity, pH 7.0: about 45% of maximal activity, hydrolysis of 4-nitrocatechol sulfate Rattus norvegicus