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Literature summary for 3.1.6.1 extracted from

  • Ueki, T.; Sawada, Y.; Fukagawa, Y.; Oki, T.
    A new type of Streptomycete arylsulfatase with high affinity to the sulfuryl moiety of the substrate (1995), Biosci. Biotechnol. Biochem., 59, 1069-1075.
    View publication on PubMed
Search for more literature for 3.1.6.1

Application

Application Comment Organism
medicine the enzyme can be accepted as the enzyme component of antitumor antibody-enzyme conjugates for target chemotherapy Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4
-
 Streptomyces sp.
1,10-phenanthroline
-
 Streptomyces sp.
2-mercaptoethanol
-
 Streptomyces sp.
AlCl3
-
 Streptomyces sp.
BaCl2
-
 Streptomyces sp.
citrate
-
 Streptomyces sp.
CoCl2
-
 Streptomyces sp.
CuCl2
-
 Streptomyces sp.
Cys
-
 Streptomyces sp.
dithiothreitol
-
 Streptomyces sp.
EDTA
-
 Streptomyces sp.
FeCl2
-
 Streptomyces sp.
Hg2+ HgCl2 Streptomyces sp.
imidazole
-
 Streptomyces sp.
iodoacetate
-
 Streptomyces sp.
K2HPO4
-
 Streptomyces sp.
K2SO3
-
 Streptomyces sp.
KCl
-
 Streptomyces sp.
MnCl2
-
 Streptomyces sp.
NaCl
-
 Streptomyces sp.
NEM
-
 Streptomyces sp.
NiCl2
-
 Streptomyces sp.
p-(amidinophenyl)methanesulfonyl fluoride HCl
-
 Streptomyces sp.
PCMB
-
 Streptomyces sp.
SO42- K2SO4 Streptomyces sp.
Zn2+ ZnCl2 Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46500
-
 1 * 46500, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
 rylsulfatase Es-2
-
Streptomyces sp. T109-3
-
 rylsulfatase Es-2
-

Purification (Commentary)

Purification (Comment) Organism
arylsulfatase Es-2 Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'',3''-di-O-acetyletoposide 4'-sulfate + H2O
-
 Streptomyces sp. 2'',3''-di-O-acetyletoposide + sulfate
-
 ?
4-acetylphenyl sulfate + H2O
-
 Streptomyces sp. 4-acetylphenol + sulfate
-
 ?
4-acetylphenyl sulfate + H2O
-
 Streptomyces sp. T109-3 4-acetylphenol + sulfate
-
 ?
4-methylumbelliferyl sulfate + H2O
-
 Streptomyces sp. 4-methylumbelliferone + sulfate
-
 ?
4-methylumbelliferyl sulfate + H2O
-
 Streptomyces sp. T109-3 4-methylumbelliferone + sulfate
-
 ?
4-nitrocatechol sulfate + H2O
-
 Streptomyces sp. 4-nitrocatechol + sulfate
-
 ?
4-nitrocatechol sulfate + H2O
-
 Streptomyces sp. T109-3 4-nitrocatechol + sulfate
-
 ?
4-nitrophenyl sulfate + H2O
-
 Streptomyces sp. 4-nitrophenol + sulfate
-
 ?
4-nitrophenyl sulfate + H2O
-
 Streptomyces sp. T109-3 4-nitrophenol + sulfate
-
 ?
chondroitin sulfate C + H2O
-
 Streptomyces sp. chondroitin + sulfate
-
 ?
chondroitin sulfate C + H2O
-
 Streptomyces sp. T109-3 chondroitin + sulfate
-
 ?
etoposide 4'-sulfate + H2O
-
 Streptomyces sp. etoposide + sulfate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 46500, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
arylsulfatase Es-2
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 enzyme Es-2 Streptomyces sp.
37
-
 enzyme Es-1 Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 50 4°C: about 40% of maximal activity, 50°C: about 25% of maximal activity, enzyme ES-2 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 enzyme Es-2, stable below Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 enzyme ES-2 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 pH 7.5: about 30% of maximal activity, pH 10: about 90% of maximal activity, enzyme Es-2 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
 rapid loss of activity below Streptomyces sp.
8 9.5 enzyme ES-2, stable Streptomyces sp.