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Literature summary for 3.1.8.1 extracted from

  • Liu, Y.H.; Liu, Y.; Chen, Z.S.; Lian, J.; Huang, X.; Chung, Y.C.
    Purification and characterization of a novel organophosphorus pesticide hydrolase from Penicillium lilacinum BP303 (2004), Enzyme Microb. Technol., 34, 297-303.
No PubMed abstract available
Search for more literature for 3.1.8.1

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 13% activation at 1 mM Purpureocillium lilacinum
dithioerythritol 20% activation at 1 mM Purpureocillium lilacinum
DTT 28% activation at 1 mM Purpureocillium lilacinum
additional information poor activation by glutathione at 1 mM Purpureocillium lilacinum
SDS 18% activation at 0.5 mM Purpureocillium lilacinum
Triton X-100 31% activation at 0.5 mM Purpureocillium lilacinum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 7% inhibition at 1 mM Purpureocillium lilacinum
4-chloromercuribenzoate complete inhibition at 0.1 mM Purpureocillium lilacinum
Fe3+ 88% inhibition at 1 mM Purpureocillium lilacinum
Hg2+ 94% inhibition at 1 mM Purpureocillium lilacinum
iodoacetic acid 92% inhibition at 0.1 mM Purpureocillium lilacinum
additional information Zn2+, Mn2+, EDTA, and Mg2+ are poor inhibitors at 1 mM Purpureocillium lilacinum
NEM complete inhibition at 0.1 mM Purpureocillium lilacinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, overview Purpureocillium lilacinum
0.033
-
 paraoxon pH 7.2, 25°C Purpureocillium lilacinum
0.052
-
 methyl parathion pH 7.2, 25°C Purpureocillium lilacinum
0.058
-
 parathion pH 7.2, 25°C Purpureocillium lilacinum
0.071
-
 Coumaphos pH 7.2, 25°C Purpureocillium lilacinum
0.187
-
 phosmet pH 7.2, 25°C Purpureocillium lilacinum
0.236
-
 demeton-S pH 7.2, 25°C Purpureocillium lilacinum
0.351
-
 malathion pH 7.2, 25°C Purpureocillium lilacinum

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Purpureocillium lilacinum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Purpureocillium lilacinum

Organism

Organism UniProt Comment Textmining
Purpureocillium lilacinum
-
-
-
Purpureocillium lilacinum BP303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native soluble enzyme 27.3fold by ultracentrifugation, ammonium sulfate precipitation, gel filtration, and ion exchange chromatography to homogeneity Purpureocillium lilacinum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Purpureocillium lilacinum
76.3
-
 purified enzyme, substrate 4-nitrophenyl phosphate at pH 7.0 and 25°C Purpureocillium lilacinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Purpureocillium lilacinum 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Purpureocillium lilacinum BP303 4-nitrophenol + phosphate
-
 ?
coumaphos + H2O
-
 Purpureocillium lilacinum 3-chloro-4-methylumbelliferone + diethyl thiophosphate
-
 ?
demeton-S + H2O
-
 Purpureocillium lilacinum 2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
 ?
malathion + H2O
-
 Purpureocillium lilacinum O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
 ?
methyl parathion + H2O
-
 Purpureocillium lilacinum 4-nitrophenol + dimethyl thiophosphate
-
 ?
methyl parathion + H2O
-
 Purpureocillium lilacinum BP303 4-nitrophenol + dimethyl thiophosphate
-
 ?
additional information substrate specificity, overview Purpureocillium lilacinum ?
-
 ?
additional information substrate specificity, overview Purpureocillium lilacinum BP303 ?
-
 ?
paraoxon + H2O
-
 Purpureocillium lilacinum 4-nitrophenol + diethyl phosphate
-
 ?
paraoxon + H2O
-
 Purpureocillium lilacinum BP303 4-nitrophenol + diethyl phosphate
-
 ?
parathion + H2O
-
 Purpureocillium lilacinum 4-nitrophenol + diethyl thiophosphate
-
 ?
parathion + H2O
-
 Purpureocillium lilacinum BP303 4-nitrophenol + diethyl thiophosphate
-
 ?
phosmet + H2O
-
 Purpureocillium lilacinum N-hydroxymethyl-phthalimide + O,O-dimethyl dithiophosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Purpureocillium lilacinum

Synonyms

Synonyms Comment Organism
OPH
-
 Purpureocillium lilacinum
organophosphorus pesticide hydrolase
-
 Purpureocillium lilacinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Purpureocillium lilacinum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 65
-
 Purpureocillium lilacinum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 3 min, purified enzyme, stable up to Purpureocillium lilacinum
50
-
 3 min, purified enzyme, about 50% remaining activity Purpureocillium lilacinum
60
-
 3 min, purified enzyme, 10% remaining activity Purpureocillium lilacinum
70
-
 3 min, purified enzyme, inactivation Purpureocillium lilacinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
160
-
 malathion pH 7.2, 25°C Purpureocillium lilacinum
650
-
 demeton-S pH 7.2, 25°C Purpureocillium lilacinum
771
-
 phosmet pH 7.2, 25°C Purpureocillium lilacinum
949
-
 Coumaphos pH 7.2, 25°C Purpureocillium lilacinum
1098
-
 parathion pH 7.2, 25°C Purpureocillium lilacinum
1152
-
 methyl parathion pH 7.2, 25°C Purpureocillium lilacinum
1336
-
 paraoxon pH 7.2, 25°C Purpureocillium lilacinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Purpureocillium lilacinum

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Purpureocillium lilacinum

pI Value

Organism Comment pI Value Maximum pI Value
Purpureocillium lilacinum isoelectric focusing
-
 4.8