Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.13.1.1 extracted from

  • Sanda, S.; Leustek, T.; Theisen, M.J.; Garavito, R.M.; Benning, C.
    Recombinant Arabidopsis SQD1 converts UDP-glucose and sulfite to the sulfolipid head group precursor UDP-sulfoquinovose in vitro (2001), J. Biol. Chem., 276, 3941-3946.
    View publication on PubMed
Search for more literature for 3.13.1.1

Activating Compound

Activating Compound Comment Organism Structure
additional information sulfate, APS, PAPS, sulfite, thiosulfate, sufide or sulfoglutathione are tested to determine whether they could stimulate the formation of UDP-sulfoquinovose Arabidopsis thaliana

Application

Application Comment Organism
medicine sulfolipids, in which synthesis the enzyme is involved, promise anti-tumor and anti-HIV therapeutics based on their inhibition of DNA polymerase and reverse transcriptase Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
T145A greatly reduced activity, site-directed mutagenesis, plays critical role for catalytic activity Arabidopsis thaliana
T145A mutant shows greatly reduced activity Arabidopsis sp.

Inhibitors

Inhibitors Comment Organism Structure
sulfite increasing concentrations beyond 0.1 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 sulfite
-
 Arabidopsis thaliana
0.01
-
 sulfite
-
 Arabidopsis sp.
0.15
-
 UDP-glucose
-
 Arabidopsis thaliana
0.15
-
 UDP-glucose
-
 Arabidopsis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast thylakoid Arabidopsis thaliana 9507
-
membrane photosynthetic Arabidopsis thaliana 16020
-
thylakoid
-
 Arabidopsis thaliana 9579
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
 recombinant protein, lacking the transit peptide Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + sulfite Arabidopsis thaliana sulfolipid biosynthesis UDP-sulfoquinovose + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis sp.
-
-
-
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-nitrilotriacetic acid column Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0026
-
-
 Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-20°C, 25 mM NaH2PO4, pH 7.5, 300 mM NaCl, 20% glycerol Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + sulfite
-
 Arabidopsis thaliana UDP-sulfoquinovose + H2O
-
 ?
UDP-glucose + sulfite sulfolipid biosynthesis Arabidopsis thaliana UDP-sulfoquinovose + H2O
-
 ?
UDP-glucose + sulfite
-
 Arabidopsis sp. UDP-6-sulfoquinovose + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
SQD1
-
 Arabidopsis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00167
-
 UDP-glucose
-
 Arabidopsis thaliana
0.1
-
 UDP-glucose
-
 Arabidopsis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.5
-
 Arabidopsis thaliana
9
-
-
 Arabidopsis sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH 7.0: about 50% of maximal activity, pH 10.0: about 35% of maximal activity Arabidopsis sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arabidopsis thaliana