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show all sequences of 3.13.1.3

Crystallization and preliminary x-ray analyses of desulfurization enzyme DszB and its C27S mutant complexed with biphenyl-2-sulfinic acid

Lee, W.C.; Ohshiro, T.; Matsubara, T.; Izumi, Y.; Tanokura, M.; Acta Crystallogr. Sect. D D60, 1636-1638 (2004)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
DszB and DszB C27S mutant complexed with biphenyl-2-sulfinic acid are crystallized. The crystals of DszB belong to the orthorhombic P2(1)2(1)2(1) space group, with unit-cell parameters a = 36.7 A, b = 82.6 A, c = 139.6 A, and contain one molecule of DszB in the asymmetric unit. Crystals of DszB C27S complexed with biphenyl-2-sulfinic acid belong to space group C2 with unit-cell parameters a = 153.4 A, b = 45.9 A, c = 112.9 A, beta = 115.93°
Rhodococcus sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus sp.
-
IGTS8
-
Rhodococcus sp. IGTS8
-
IGTS8
-
Purification (Commentary)
Commentary
Organism
-
Rhodococcus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(2-hydroxyphenyl)benzenesulfinate + H2O
-
657474
Rhodococcus sp.
2-hydroxybiphenyl + sulfite
-
-
-
?
2-(2-hydroxyphenyl)benzenesulfinate + H2O
-
657474
Rhodococcus sp. IGTS8
2-hydroxybiphenyl + sulfite
-
-
-
?
Crystallization (Commentary) (protein specific)
Crystallization
Organism
DszB and DszB C27S mutant complexed with biphenyl-2-sulfinic acid are crystallized. The crystals of DszB belong to the orthorhombic P2(1)2(1)2(1) space group, with unit-cell parameters a = 36.7 A, b = 82.6 A, c = 139.6 A, and contain one molecule of DszB in the asymmetric unit. Crystals of DszB C27S complexed with biphenyl-2-sulfinic acid belong to space group C2 with unit-cell parameters a = 153.4 A, b = 45.9 A, c = 112.9 A, beta = 115.93°
Rhodococcus sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rhodococcus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(2-hydroxyphenyl)benzenesulfinate + H2O
-
657474
Rhodococcus sp.
2-hydroxybiphenyl + sulfite
-
-
-
?
2-(2-hydroxyphenyl)benzenesulfinate + H2O
-
657474
Rhodococcus sp. IGTS8
2-hydroxybiphenyl + sulfite
-
-
-
?
Other publictions for EC 3.13.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695786
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1
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1
-
2
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1
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-
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-
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-
-
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2
1
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2
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-
1
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2
-
1
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-
-
695760
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Rhodococcus erythropolis
Appl. Environ. Microbiol.
74
971-976
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2
1
-
-
-
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-
1
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1
-
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2
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2
1
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1
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2
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683271
Ohshiro
Improvement of 2-hydroxybiphen ...
Rhodococcus erythropolis, Rhodococcus erythropolis IGTS8 / ATCC 53968
Biosci. Biotechnol. Biochem.
71
2815-2821
2007
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-
1
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7
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-
4
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-
-
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3
-
-
-
-
-
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
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-
4
-
-
-
-
-
-
-
-
-
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
669500
Lee
Crystal structure and desulfur ...
Rhodococcus erythropolis
J. Biol. Chem.
281
32534-32539
2006
-
-
-
1
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657474
Lee
Crystallization and preliminar ...
Rhodococcus sp., Rhodococcus sp. IGTS8
Acta Crystallogr. Sect. D
D60
1636-1638
2004
-
-
-
1
-
-
-
-
-
-
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-
-
2
-
-
1
-
-
-
-
-
2
-
-
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-
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-
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-
-
-
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-
-
-
1
-
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-
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-
-
-
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-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649406
Konishi
2-(2'-Hydroxyphenyl)benzene su ...
Paenibacillus sp., Paenibacillus sp. A11-2
Appl. Microbiol. Biotechnol.
62
356-361
2003
1
-
1
-
1
-
12
1
-
-
2
2
-
6
-
-
1
-
-
-
1
-
6
1
1
-
2
-
1
-
-
1
-
-
-
1
-
1
1
-
1
-
-
12
-
1
-
-
2
2
-
-
-
1
-
-
1
-
6
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
649487
Watkins
Purification and characterizat ...
Rhodococcus erythropolis, Rhodococcus erythropolis IGTS8 / ATCC 53968
Arch. Biochem. Biophys.
415
14-23
2003
2
-
-
-
-
-
10
1
-
-
1
2
-
8
-
-
1
-
-
-
1
-
6
1
1
1
-
-
1
1
-
-
-
1
-
2
-
-
-
-
-
-
-
10
-
1
-
-
1
2
-
-
-
1
-
-
1
-
6
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
650387
Nakayama
A novel enzyme, 2'-hydroxybiph ...
Rhodococcus erythropolis, Rhodococcus erythropolis KA2-5-1
Biochim. Biophys. Acta
1598
122-130
2002
1
-
1
-
1
-
10
2
-
1
2
2
-
5
-
-
1
1
-
-
2
-
6
1
1
-
2
-
1
-
-
1
3
-
-
1
-
1
1
-
1
-
-
10
3
2
-
1
2
2
-
-
-
1
-
-
2
-
6
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
208354
Oldfield
Elucidation of the metabolic p ...
Rhodococcus rhodochrous, Rhodococcus sp. IGTS8 / ATCC 53968
Microbiology
143
2961-2973
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
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-
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-
-
-
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-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
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-
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-
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