BRENDA - Enzyme Database show
show all sequences of 3.13.1.3

Crystal structure and desulfurization mechanism of 2'-hydroxybiphenyl-2-sulfinic acid desulfinase

Lee, W.C.; Ohshiro, T.; Matsubara, T.; Izumi, Y.; Tanokura, M.; J. Biol. Chem. 281, 32534-32539 (2006)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
crystal structure of DszB and an inactive mutant of DszB in complex with substrate at resolution of 1.8 A or better
Rhodococcus erythropolis
Engineering
Amino acid exchange
Commentary
Organism
H60Q
mutation causes an 17fold reduction in the specific activity
Rhodococcus erythropolis
R70I
inactive mutant mostly exists in the insoluble fraction of the cell extract
Rhodococcus erythropolis
R70K
inactive mutant mostly exists in the insoluble fraction of the cell extract
Rhodococcus erythropolis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus erythropolis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-hydroxybiphenyl-2-sulfinate + H2O
-
669500
Rhodococcus erythropolis
2-hydroxybiphenyl + sulfite
-
-
-
?
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystal structure of DszB and an inactive mutant of DszB in complex with substrate at resolution of 1.8 A or better
Rhodococcus erythropolis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H60Q
mutation causes an 17fold reduction in the specific activity
Rhodococcus erythropolis
R70I
inactive mutant mostly exists in the insoluble fraction of the cell extract
Rhodococcus erythropolis
R70K
inactive mutant mostly exists in the insoluble fraction of the cell extract
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-hydroxybiphenyl-2-sulfinate + H2O
-
669500
Rhodococcus erythropolis
2-hydroxybiphenyl + sulfite
-
-
-
?
Other publictions for EC 3.13.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695786
Calzada
Analysis of dibenzothiophene d ...
Pseudomonas putida, Pseudomonas putida CECT 5279
Appl. Environ. Microbiol.
75
875-877
2009
-
2
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
695760
Li
Genetic rearrangement strategy ...
Rhodococcus erythropolis
Appl. Environ. Microbiol.
74
971-976
2008
-
2
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683271
Ohshiro
Improvement of 2-hydroxybiphen ...
Rhodococcus erythropolis, Rhodococcus erythropolis IGTS8 / ATCC 53968
Biosci. Biotechnol. Biochem.
71
2815-2821
2007
-
-
1
-
7
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
669500
Lee
Crystal structure and desulfur ...
Rhodococcus erythropolis
J. Biol. Chem.
281
32534-32539
2006
-
-
-
1
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657474
Lee
Crystallization and preliminar ...
Rhodococcus sp., Rhodococcus sp. IGTS8
Acta Crystallogr. Sect. D
D60
1636-1638
2004
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649406
Konishi
2-(2'-Hydroxyphenyl)benzene su ...
Paenibacillus sp., Paenibacillus sp. A11-2
Appl. Microbiol. Biotechnol.
62
356-361
2003
1
-
1
-
1
-
12
1
-
-
2
2
-
6
-
-
1
-
-
-
1
-
6
1
1
-
2
-
1
-
-
1
-
-
-
1
-
1
1
-
1
-
-
12
-
1
-
-
2
2
-
-
-
1
-
-
1
-
6
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
649487
Watkins
Purification and characterizat ...
Rhodococcus erythropolis, Rhodococcus erythropolis IGTS8 / ATCC 53968
Arch. Biochem. Biophys.
415
14-23
2003
2
-
-
-
-
-
10
1
-
-
1
2
-
8
-
-
1
-
-
-
1
-
6
1
1
1
-
-
1
1
-
-
-
1
-
2
-
-
-
-
-
-
-
10
-
1
-
-
1
2
-
-
-
1
-
-
1
-
6
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
650387
Nakayama
A novel enzyme, 2'-hydroxybiph ...
Rhodococcus erythropolis, Rhodococcus erythropolis KA2-5-1
Biochim. Biophys. Acta
1598
122-130
2002
1
-
1
-
1
-
10
2
-
1
2
2
-
5
-
-
1
1
-
-
2
-
6
1
1
-
2
-
1
-
-
1
3
-
-
1
-
1
1
-
1
-
-
10
3
2
-
1
2
2
-
-
-
1
-
-
2
-
6
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
208354
Oldfield
Elucidation of the metabolic p ...
Rhodococcus rhodochrous, Rhodococcus sp. IGTS8 / ATCC 53968
Microbiology
143
2961-2973
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-