Any feedback?
Literature summary for 3.2.1.1 extracted from
- Mechanistic analyses of catalysis in human pancreatic alpha-amylase: detailed kinetic and structural studies of mutants of three conserved carboxylic acids (2002), Biochemistry, 41, 4492-4502.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D197A | no activity | Homo sapiens |
| D197N | no activity | Homo sapiens |
| D300A | 0.0005% of wild-type starch hydrolyzing activity | Homo sapiens |
| D300N | 0.0005% of wild-type starch hydrolyzing activity | Homo sapiens |
| E233A | 0.005% of wild-type starch hydrolyzing activity | Homo sapiens |
| E233A/D300A | 0.001% of wild-type starch hydrolyzing activity | Homo sapiens |
| E233Q | 0.0005% of wild-type starch hydrolyzing activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.51 | - |
alpha-maltotriosyl fluoride | 30°C, pH 7.0 | Homo sapiens |  |
| 4.5 | - |
alpha-maltosyl fluoride | 30°C, pH 7.0 | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56091 | - |
x * 56091, deduced from nucloeotide sequence | Homo sapiens |
| 56095 | - |
x * 56095, mass spectrometry | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Homo sapiens | - |
maltooligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant alpha-amylase, Phenyl Sepharose, Q-Sepharose | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5500 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-maltosyl fluoride + H2O | - |
Homo sapiens | maltose + fluoride | - |
? | |
| alpha-maltotriosyl fluoride + H2O | - |
Homo sapiens | maltotriose + fluoride | - |
? | |
| maltopentaose + H2O | - |
Homo sapiens | maltotriose + maltose | major products | ? | |
| starch + H2O | - |
Homo sapiens | maltooligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56091, deduced from nucloeotide sequence | Homo sapiens |
| ? | x * 56095, mass spectrometry | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HPA | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 283 | - |
alpha-maltotriosyl fluoride | 30°C, pH 7.0 | Homo sapiens | |
| 443 | - |
alpha-maltosyl fluoride | 30°C, pH 7.0 | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | hydrolysis of alpha-maltotriosyl fluoride | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
