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Literature summary for 3.2.1.1 extracted from

  • Wanderley, K.J.; Torres, F.A.; Moraes, L.M.; Ulhoa, C.J.
    Biochemical characterization of alpha-amylase from the yeast Cryptococcus flavus (2004), FEMS Microbiol. Lett., 231, 165-169.
    View publication on PubMed
Search for more literature for 3.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 4 mM, 30% inhibition Saitozyma flava
Cu2+
-
 Saitozyma flava
CuSO4 4 mM, complete inhibition Saitozyma flava
Fe2+
-
 Saitozyma flava
FeSO4 4 mM, 80% inhibition Saitozyma flava
Hg2+
-
 Saitozyma flava
HgCl2 4 mM, 71% inhibition Saitozyma flava
additional information treatment with glycosidases leads to loss of 50% activity Saitozyma flava

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 starch 0.056 mg/ml, 50°C, pH 5.5 Saitozyma flava
0.057
-
 soluble starch pH 5.5, 50°C Saitozyma flava

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Saitozyma flava
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 1 * 75000, SDS-PAGE Saitozyma flava
84500
-
 gel filtration Saitozyma flava

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Saitozyma flava
-
 maltose + maltotriose
-
 ?

Organism

Organism UniProt Comment Textmining
Saitozyma flava
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein highly glycosylated Saitozyma flava
glycoprotein treatment with glycosidases leads to loss of 50% activity Saitozyma flava

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme from culture medium 16.14fold in a single gel filtration step Saitozyma flava
Sephacryl S-100 Saitozyma flava

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 842.9 units, one unit is defined as the amount of alpha-amylase necessary to hydrolyze 0.1 mg starch per min Saitozyma flava

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Saitozyma flava maltose + maltotriose
-
 ?
amylopectin + H2O
-
 Saitozyma flava maltose + maltotriose main products ?
amylose + H2O
-
 Saitozyma flava maltose + maltotriose
-
 ?
amylose + H2O
-
 Saitozyma flava maltose + maltotriose main products ?
glycogen + H2O
-
 Saitozyma flava maltose + maltotriose main products ?
glycogen starch + H2O
-
 Saitozyma flava maltose + maltotriose
-
 ?
pullulan + H2O
-
 Saitozyma flava maltose + maltotriose main products ?
starch + H2O
-
 Saitozyma flava maltose + maltotriose
-
 ?
starch + H2O soluble starch Saitozyma flava maltose + maltotriose main products ?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE Saitozyma flava

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Saitozyma flava

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10% loss of activity after 1 h Saitozyma flava
50
-
 60 min, purified enzyme, 90% remaining activity Saitozyma flava
60
-
 40% loss of activity after 1 h Saitozyma flava

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Saitozyma flava