Cloned (Comment) | Organism |
---|---|
expression of AHA mutants in Escherichia coli | Pseudoalteromonas haloplanktis |
Protein Variants | Comment | Organism |
---|---|---|
K300R | 47% of wild-type kcat | Pseudoalteromonas haloplanktis |
K300R/N150D/V196F/Q164I/T232V | 44% of wild-type kcat, increase in temperature stability | Pseudoalteromonas haloplanktis |
N150D | 98% of wild-type kcat | Pseudoalteromonas haloplanktis |
Q164I | 73% of wild-type kcat | Pseudoalteromonas haloplanktis |
T232V | 105% of wild-type kcat | Pseudoalteromonas haloplanktis |
V196F | 108% of wild-type kcat | Pseudoalteromonas haloplanktis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 25°C, pH 7.2 | Sus scrofa | |
0.23 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 25°C, pH 7.2 | Pseudoalteromonas haloplanktis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Sus scrofa | - |
maltooligosaccharides | - |
? | |
starch + H2O | Pseudoalteromonas haloplanktis | - |
maltooligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas haloplanktis | - |
- |
- |
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Sus scrofa | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
starch + H2O | - |
Sus scrofa | maltooligosaccharides | - |
? | |
starch + H2O | - |
Pseudoalteromonas haloplanktis | maltooligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AHA | - |
Pseudoalteromonas haloplanktis |
PPA | - |
Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
wild-type, approx. 70% loss of activity after 45 min in the absence of starch, approx. 95% loss of activity after 45 min in the presence of 2% starch, K300R/N150D/V196F/Q164I/T232V mutant, approx. 50% loss of activity in the absence of starch | Pseudoalteromonas haloplanktis |
60 | - |
approx. 50% loss of activity after 30 min in the presence of 2% starch, approx. 85% loss of activity after 30 min in the absence of starch | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
141 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 25°C, pH 7.2 | Sus scrofa | |
291 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 25°C, pH 7.2 | Sus scrofa | |
392 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 15°C, pH 7.2 | Pseudoalteromonas haloplanktis | |
697 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 25°C, pH 7.2 | Pseudoalteromonas haloplanktis |