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Literature summary for 3.2.1.1 extracted from
- Characterization of a salt-tolerant extracellular alpha-amylase from Bacillus dipsosauri (2002), Lett. Appl. Microbiol., 35, 78-84.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CdCl2 | 30 mM, 95% inhibition | Gracilibacillus dipsosauri |  |
| CoCl2 | 30 mM, 50% inhibition | Gracilibacillus dipsosauri | |
| maltopentaose | 30 mM, 51% inhibition | Gracilibacillus dipsosauri | |
| maltotetraose | 30 mM, 51% inhibition | Gracilibacillus dipsosauri | |
| maltotriose | 30 mM, 42% inhibition | Gracilibacillus dipsosauri | |
| NiCl2 | 30 mM, 39% inhibition | Gracilibacillus dipsosauri | |
| ZnCl2 | 30 mM, complete inhibition | Gracilibacillus dipsosauri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.5 | - |
2-chloro-4-nitrophenyl-alpha-D-maltotrioside | 22°C, pH 6.1 | Gracilibacillus dipsosauri | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Gracilibacillus dipsosauri | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na2SO4 | 1 M, 4fold increase in activity | Gracilibacillus dipsosauri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
native PAGE | Gracilibacillus dipsosauri |
| 80000 | - |
gel filtration | Gracilibacillus dipsosauri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Gracilibacillus dipsosauri | - |
fragments of starch | - |
? | |
| starch + H2O | Gracilibacillus dipsosauri DD1 | - |
fragments of starch | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gracilibacillus dipsosauri | - |
moderately halophilic bacterium | - |
| Gracilibacillus dipsosauri DD1 | - |
moderately halophilic bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ethanol, DEAE-cellulose, BioGel P-100 | Gracilibacillus dipsosauri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM 2-[N-morpholino]ethanesulfonic acid, pH 6.1, several months, no loss of activity | Gracilibacillus dipsosauri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-chloro-4-nitrophenyl-alpha-D-maltotrioside + H2O | - |
Gracilibacillus dipsosauri | 2-chloro-4-nitrophenol + maltotriose | - |
? | |
| 2-chloro-4-nitrophenyl-alpha-D-maltotrioside + H2O | - |
Gracilibacillus dipsosauri DD1 | 2-chloro-4-nitrophenol + maltotriose | - |
? | |
| starch + H2O | - |
Gracilibacillus dipsosauri | fragments of starch | - |
? | |
| starch + H2O | - |
Gracilibacillus dipsosauri DD1 | fragments of starch | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 80000, SDS-PAGE | Gracilibacillus dipsosauri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Gracilibacillus dipsosauri |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | approx. 30% of maximal activity at 25°C, approx. 90% of maximal activity at 55°C, no activity at 65°C | Gracilibacillus dipsosauri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
in the presence of 1 M KCl | Gracilibacillus dipsosauri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | approx. 50% of maximal activity at pH 5.5 and pH 8.0 | Gracilibacillus dipsosauri |
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