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Literature summary for 3.2.1.1 extracted from
- Purification and characterization of two alkaline, thermotolerant alpha-amylases from Bacillus halodurans 38C-2-1 and expression of the cloned gene in Escherichia coli (2007), Biosci. Biotechnol. Biochem., 71, 2393-2401.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, alpha-amylase I | Halalkalibacterium halodurans |
| expression in Escherichia coli, alpha-amylase II | Halalkalibacterium halodurans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | 1 mM, 40°C, 30 min, 12% loss of activity, alpha-amylase I | Halalkalibacterium halodurans |  |
| BaCl2 | 1 mM, 40°C, 30 min, 16% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
| CoCl2 | 1 mM, 40°C, 30 min, 22% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
| CuSO4 | 1 mM, 40°C, 30 min, 25% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
| EDTA | 1 mM, 40°C, 30 min, complete loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
| FeCl3 | 1 mM, 40°C, 30 min, 21% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
| FeSO4 | 1 mM, 40°C, 30 min, 86% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
| HgCl2 | 1 mM, 40°C, 30 min, 95% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
| MnCl2 | 1 mM, 40°C, 30 min, 46% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
| NiSO4 | 1 mM, 40°C, 30 min, 79% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
| PbCl2 | 1 mM, 40°C, 30 min, 35% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
| ZnSO4 | 1 mM, 40°C, 30 min, 47% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 10000 | - |
alpha-amylase I, non-denaturing PAGE | Halalkalibacterium halodurans |
| 75000 | - |
1 * 75000, alpha-amylase II, SDS-PAGE | Halalkalibacterium halodurans |
| 87000 | - |
alpha-amylase II, non-denaturing PAGE | Halalkalibacterium halodurans |
| 100000 | - |
1 * 100000, alpha-amylase I, SDS-PAGE | Halalkalibacterium halodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halalkalibacterium halodurans | - |
- |
- |
| Halalkalibacterium halodurans 38C-2-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| alpha-amylase I | Halalkalibacterium halodurans |
| alpha-amylase II | Halalkalibacterium halodurans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18 | - |
alpha-amylase II | Halalkalibacterium halodurans |
| 130 | - |
alpha-amylase I | Halalkalibacterium halodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | 121% of the activity with soluble starch | Halalkalibacterium halodurans | ? | - |
? | |
| amylopectin + H2O | 121% of the activity with soluble starch | Halalkalibacterium halodurans 38C-2-1 | ? | - |
? | |
| amylose + H2O | 30% of the activity with soluble starch | Halalkalibacterium halodurans | ? | - |
? | |
| amylose + H2O | 30% of the activity with soluble starch | Halalkalibacterium halodurans 38C-2-1 | ? | - |
? | |
| glycogen + H2O | 104% of the activity with soluble starch | Halalkalibacterium halodurans | ? | - |
? | |
| glycogen + H2O | 104% of the activity with soluble starch | Halalkalibacterium halodurans 38C-2-1 | ? | - |
? | |
| additional information | no hydrolytic activity towards pullulan or dextran | Halalkalibacterium halodurans | ? | - |
? | |
| additional information | no hydrolytic activity towards pullulan or dextran | Halalkalibacterium halodurans 38C-2-1 | ? | - |
? | |
| soluble starch + H2O | - |
Halalkalibacterium halodurans | ? | - |
? | |
| soluble starch + H2O | - |
Halalkalibacterium halodurans 38C-2-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 100000, alpha-amylase I, SDS-PAGE | Halalkalibacterium halodurans |
| monomer | 1 * 75000, alpha-amylase II, SDS-PAGE | Halalkalibacterium halodurans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-amylase I | - |
Halalkalibacterium halodurans |
| alpha-amylase II | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
alpha-amylase II | Halalkalibacterium halodurans |
| 50 | 60 | alpha-amylase I | Halalkalibacterium halodurans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: 38% of maximal activity, 50-60: maximal activity, alpha-amylase II | Halalkalibacterium halodurans |
| 30 | 60 | 30°C: 42% of maximal activity, 50-60°C: maximal activity, alpha-amylase I | Halalkalibacterium halodurans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, 58% loss of activity, alpha-amylase II | Halalkalibacterium halodurans |
| 50 | - |
30 min, less than 5% loss of activity, alpha-amylase I | Halalkalibacterium halodurans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
alpha-amylase I | Halalkalibacterium halodurans |
| 10 | 11 | alpha-amylase I | Halalkalibacterium halodurans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | 4°C, 24 h, stable, alpha-amylase I | Halalkalibacterium halodurans |
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