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Literature summary for 3.2.1.1 extracted from

  • Kato, S.; Shimizu-Ibuka, A.; Mura, K.; Takeuchi, A.; Tokue, C.; Arai, S.
    Molecular cloning and characterization of an alpha-amylase from Pichia burtonii 15-1 (2007), Biosci. Biotechnol. Biochem., 71, 3007-3013.
    View publication on PubMed
Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Hyphopichia burtonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 starch pH 5.6, 40°C Hyphopichia burtonii
0.48
-
 maltotetraose pH 5.6, 40°C Hyphopichia burtonii
0.55
-
 maltopentaose pH 5.6, 40°C Hyphopichia burtonii
0.71
-
 maltohexaose pH 5.6, 40°C Hyphopichia burtonii
2.35
-
 gamma-cyclodextrin pH 5.6, 40°C Hyphopichia burtonii
4.13
-
 pullulan pH 5.6, 40°C Hyphopichia burtonii
6.4
-
 maltose pH 5.6, 40°C Hyphopichia burtonii
12.3
-
 beta-cyclodextrin pH 5.6, 40°C Hyphopichia burtonii
14.9
-
 alpha-cyclodextrin pH 5.6, 40°C Hyphopichia burtonii
28.6
-
 maltotriose pH 5.6, 40°C Hyphopichia burtonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51750
-
 x * 51750, calculated from sequence Hyphopichia burtonii

Organism

Organism UniProt Comment Textmining
Hyphopichia burtonii
-
-
-
Hyphopichia burtonii 15-1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + H2O weak activity Hyphopichia burtonii ?
-
 ?
beta-cyclodextrin + H2O
-
 Hyphopichia burtonii ?
-
 ?
beta-cyclodextrin + H2O
-
 Hyphopichia burtonii 15-1 ?
-
 ?
gamma-cyclodextrin + H2O
-
 Hyphopichia burtonii ?
-
 ?
maltohexaose + H2O
-
 Hyphopichia burtonii ?
-
 ?
maltohexaose + H2O
-
 Hyphopichia burtonii 15-1 ?
-
 ?
maltopentaose + H2O
-
 Hyphopichia burtonii ?
-
 ?
maltopentaose + H2O
-
 Hyphopichia burtonii 15-1 ?
-
 ?
maltose + H2O weak activity Hyphopichia burtonii 2 D-glucose
-
 ?
maltotetraose + H2O
-
 Hyphopichia burtonii ?
-
 ?
maltotetraose + H2O
-
 Hyphopichia burtonii 15-1 ?
-
 ?
maltotriose + H2O low activity Hyphopichia burtonii ?
-
 ?
pullulan + H2O
-
 Hyphopichia burtonii ?
-
 ?
pullulan + H2O
-
 Hyphopichia burtonii 15-1 ?
-
 ?
starch + H2O
-
 Hyphopichia burtonii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51750, calculated from sequence Hyphopichia burtonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 maltotetraose pH 5.6, 40°C Hyphopichia burtonii
0.0258
-
 maltose pH 5.6, 40°C Hyphopichia burtonii
0.085
-
 pullulan pH 5.6, 40°C Hyphopichia burtonii
0.24
-
 maltotriose pH 5.6, 40°C Hyphopichia burtonii
0.399
-
 alpha-cyclodextrin pH 5.6, 40°C Hyphopichia burtonii
5.87
-
 maltotetraose pH 5.6, 40°C Hyphopichia burtonii
31.1
-
 beta-cyclodextrin pH 5.6, 40°C Hyphopichia burtonii
37.2
-
 maltopentaose pH 5.6, 40°C Hyphopichia burtonii
44.2
-
 maltohexaose pH 5.6, 40°C Hyphopichia burtonii
64.6
-
 gamma-cyclodextrin pH 5.6, 40°C Hyphopichia burtonii
91.4
-
 starch pH 5.6, 40°C Hyphopichia burtonii