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Literature summary for 3.2.1.1 extracted from
- Gene cloning and characterization of a novel alpha-amylase from alkaliphilic Alkalimonas amylolytica (2006), Biotechnol. J., 1, 1258-1265.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Alkalimonas amylolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 5 mM, weak inhibition | Alkalimonas amylolytica |  |
| Ca2+ | 5 mM, weak inhibition | Alkalimonas amylolytica | |
| Cu2+ | 5 mM, complete inhibition | Alkalimonas amylolytica | |
| Hg+ | 5 mM, complete inhibition | Alkalimonas amylolytica | |
| iodoacetic acid | 5 mM | Alkalimonas amylolytica | |
| Mn2+ | 5 mM, complete inhibition | Alkalimonas amylolytica | |
| N-bromosuccinimide | 1 mM | Alkalimonas amylolytica | |
| NaCl | 83% of the initial activity remains in 2 M NaCl | Alkalimonas amylolytica | |
| Pb2+ | 5 mM, complete inhibition | Alkalimonas amylolytica | |
| SDS | with 0.1%, 0.2% and 0.5% SDS, the enzyme shows 64%, 43% and 33% activity as compared with the activity in the absence of SDS | Alkalimonas amylolytica | |
| Zn2+ | 5 mM, weak inhibition | Alkalimonas amylolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km values: about 0.5 mg/ml for soluble starch and 4.45 mg/ml for glycogen at pH 9.5 and 50°C | Alkalimonas amylolytica |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Alkalimonas amylolytica | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5 mM, strong stimulation | Alkalimonas amylolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
x * 61000, SDS-PAGE | Alkalimonas amylolytica |
| 61160 | - |
x * 61160, calculated from sequence | Alkalimonas amylolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alkalimonas amylolytica | Q6WUB6 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7826 | - |
- |
Alkalimonas amylolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | 69% of the activity with soluble starch | Alkalimonas amylolytica | ? | - |
? | |
| amylose + H2O | 61% of the activity with soluble starch | Alkalimonas amylolytica | ? | - |
? | |
| glycogen + H2O | 32% of the activity with soluble starch | Alkalimonas amylolytica | ? | - |
? | |
| additional information | the enzyme has some glycosyl transferase activity | Alkalimonas amylolytica | ? | - |
? | |
| soluble starch + H2O | - |
Alkalimonas amylolytica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000, SDS-PAGE | Alkalimonas amylolytica |
| ? | x * 61160, calculated from sequence | Alkalimonas amylolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AMY1 | - |
Alkalimonas amylolytica |
| AmyA | - |
Alkalimonas amylolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Alkalimonas amylolytica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | more than 70% of the maximum activity is detectable between 45°C and 55°C | Alkalimonas amylolytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
30 min, stable up to | Alkalimonas amylolytica |
| 50 | - |
30 min, 29% loss of activity | Alkalimonas amylolytica |
| 55 | - |
30 min, unstable above | Alkalimonas amylolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Alkalimonas amylolytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10.5 | more than 50% of the maximum activity is detectable between pH 8 and 10.5 | Alkalimonas amylolytica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 11 | 40°C, 60 min, enzyme retains almost 80% of its initial activity | Alkalimonas amylolytica |
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