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Literature summary for 3.2.1.1 extracted from
- Purification and properties of midgut alpha-amylase isolated from Morimus funereus (Coleoptera: Cerambycidae) larvae (2008), Comp. Biochem. Physiol. B, 149, 153-160.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| amylase wheat inhibitor | - |
Morimus funereus | |
| CaCl2 | above 0.1 mM | Morimus funereus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for soluble starch: 0.43 mg/ml | Morimus funereus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | maximal activity at 0.1 mM, higher concentrations inhibit activity | Morimus funereus | |
| NaCl | activation above 10 mM, 28% activation at 250 mM | Morimus funereus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
gel filtration | Morimus funereus |
| 33000 | - |
1 * 33000, SDS-PAGE | Morimus funereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Morimus funereus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Morimus funereus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | midgut | Morimus funereus | - |
| midgut | larva | Morimus funereus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1068 | - |
- |
Morimus funereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| soluble starch + H2O | maximal activity is achieved with horse-radish starch, undetectable activity towards potato starch | Morimus funereus | maltose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33000, SDS-PAGE | Morimus funereus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AMF-3 | - |
Morimus funereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Morimus funereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
complete inactivation | Morimus funereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Morimus funereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | 6.7 | pH 4.2: about 70% of maximal activity, pH 6.7: about 35% of maximal activity | Morimus funereus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Morimus funereus | isoelectric focusing | - |
3.2 |
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