BRENDA - Enzyme Database show
show all sequences of 3.2.1.1

Comparison of the wild-type alpha-amylase and its variant enzymes in Bacillus amyloliquefaciens in activity and thermal stability, and insights into engineering the thermal stability of Bacillus alpha-amylase

Lee, S.; Mouri, Y.; Minoda, M.; Oneda, H.; Inouye, K.; J. Biochem. 139, 1007-1015 (2006)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
0.24 mg/ml for soluble starch, variant enzyme M77; 0.34 mg/ml for soluble starch, wild-type enzyme; 0.63 mg/ml for soluble starch, variant enzyme 21B; 0.8 mg/ml for soluble starch, variant enzyme M111
Bacillus amyloliquefaciens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus amyloliquefaciens
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
soluble starch + H2O
-
680534
Bacillus amyloliquefaciens
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
optimal temperature in absence of Ca2+, variant enzyme 21B; optimal temperature in absence of Ca2+, variant enzyme M111
Bacillus amyloliquefaciens
50
60
optimal temperature in presence of 5 mM Ca2+, variant enzyme 21B; optimal temperature in presence of 5 mM Ca2+, variant enzyme M111
Bacillus amyloliquefaciens
60
-
optimal temperature in absence of Ca2+ and presence of 5 mM Ca2+, variant enzyme M77; optimal temperature in absence of Ca2+ and presence of 5 mM Ca2+, wild-type enzyme
Bacillus amyloliquefaciens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
503 per s for soluble starch, variant enzyme M77; 586 per s for soluble starch, wild-type enzyme; 656 per s for soluble starch, variant enzyme M111; 722 per s for soluble starch, variant enzyme 21B
Bacillus amyloliquefaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
variant enzyme 21B; variant enzyme M111; variant enzyme M77; wild-type enzyme
Bacillus amyloliquefaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
0.24 mg/ml for soluble starch, variant enzyme M77; 0.34 mg/ml for soluble starch, wild-type enzyme; 0.63 mg/ml for soluble starch, variant enzyme 21B; 0.8 mg/ml for soluble starch, variant enzyme M111
Bacillus amyloliquefaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
soluble starch + H2O
-
680534
Bacillus amyloliquefaciens
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
optimal temperature in absence of Ca2+, variant enzyme 21B; optimal temperature in absence of Ca2+, variant enzyme M111
Bacillus amyloliquefaciens
50
60
optimal temperature in presence of 5 mM Ca2+, variant enzyme 21B; optimal temperature in presence of 5 mM Ca2+, variant enzyme M111
Bacillus amyloliquefaciens
60
-
optimal temperature in absence of Ca2+ and presence of 5 mM Ca2+, variant enzyme M77; optimal temperature in absence of Ca2+ and presence of 5 mM Ca2+, wild-type enzyme
Bacillus amyloliquefaciens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
503 per s for soluble starch, variant enzyme M77; 586 per s for soluble starch, wild-type enzyme; 656 per s for soluble starch, variant enzyme M111; 722 per s for soluble starch, variant enzyme 21B
Bacillus amyloliquefaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
variant enzyme 21B; variant enzyme M111; variant enzyme M77; wild-type enzyme
Bacillus amyloliquefaciens
Other publictions for EC 3.2.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)