Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activator at concentrations below 150 mM, activating only a maximum of 3% at 100 mM, and a slight inhibitor at higher concentrations (8% of the initial activity at 200 mM) | Haloferax mediterranei |
General Stability | Organism |
---|---|
the enzyme is stable from 2 to 4 M NaCl | Haloferax mediterranei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | pH 7.5, irreversible loss of activity, half-life: 29 h at 25 mM, 64 h at 12 mM, 670 h at 6 mM and 3500 at 3 mM | Haloferax mediterranei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | when this medium is enriched with starch, alpha-amylase activity is excreted to the medium in low concentration | Haloferax mediterranei | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activator at concentrations below 150 mM, activating only a maximum of 3% at 100 mM, and a slight inhibitor at higher concentrations (8% of the initial activity at 200 mM) | Haloferax mediterranei | |
Mg2+ | inhibitor when added in concentrations from 50 mM to 200 mM. The maximum inhibitory effect is obtained at 100 mM, inhibiting only 10% of the initial activity | Haloferax mediterranei | |
NaCl | maximal activity at 3 M NaCl | Haloferax mediterranei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Haloferax mediterranei |
58000 | - |
1 * 58000, SDS-PAGE | Haloferax mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloferax mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | the activity with alpha-cyclodextrin is 46% of the activity with starch in the same conditions at 0.5% (w/v) substrate concentrations | Haloferax mediterranei | ? | - |
? | |
beta-cyclodextrin + H2O | the activity with alpha-cyclodextrin is 48% of the activity with starch in the same conditions at 0.5% (w/v) substrate | Haloferax mediterranei | ? | - |
? | |
starch + H2O | endo-acting activity | Haloferax mediterranei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, SDS-PAGE | Haloferax mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Haloferax mediterranei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | 50-60: temperature optimum, 80°C: 65% of maximal activity | Haloferax mediterranei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 7.0, half-life: 2100 h | Haloferax mediterranei |
50 | - |
pH 7.0, half-life: 10.1 h | Haloferax mediterranei |
60 | - |
pH 7.0, half-life: 0.88 h | Haloferax mediterranei |
70 | - |
pH 7.0, half-life: 7.2 min | Haloferax mediterranei |
80 | - |
pH 7.0, half-life: 4.1 min | Haloferax mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Haloferax mediterranei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
at 3 M NaCl, half-life: 5.7 h | Haloferax mediterranei |
4.6 | - |
at 3 M NaCl, half-life: 138 h | Haloferax mediterranei |
5 | - |
at 3 M NaCl, half-life: 15000 h | Haloferax mediterranei |
6 | 8 | at 3 M NaCl, half-life: 100 d | Haloferax mediterranei |
9 | - |
at 3 M NaCl, half-life: 280 d | Haloferax mediterranei |
10 | - |
at 3 M NaCl, half-life: 240 d | Haloferax mediterranei |