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Literature summary for 3.2.1.1 extracted from
- Characterization and comparative analysis of psychrophilic and mesophilic alpha-amylases from Euplotes species: a contribution to the understanding of enzyme thermal adaptation (2013), Biochem. Biophys. Res. Commun., 438, 715-720.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Euplotes focardii |
| expression in Escherichia coli | Moneuplotes crassus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
starch | KM value is 1.31 g/l at 5°C, 1.36 g/l at 25°C, and 1.42 g/l at 35°C respectively, and pH 9.0 | Moneuplotes crassus |  |
| additional information | - |
starch | KM value is 3 g/l at 5°C, 3.3g/l at 25°C, and 4.4 g/l at 35°C respectively, and pH 9.0 | Euplotes focardii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
x * 52000, SDS-PAGE | Euplotes focardii |
| 53000 | - |
x * 53000, SDS-PAGE | Moneuplotes crassus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euplotes focardii | T1WE96 | - |
- |
| Moneuplotes crassus | T1WDH2 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Euplotes focardii | ? | - |
? | |
| starch + H2O | - |
Moneuplotes crassus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Moneuplotes crassus |
| ? | x * 52000, SDS-PAGE | Euplotes focardii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 25 | - |
Euplotes focardii |
| 35 | - |
- |
Moneuplotes crassus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
50% of maximum activity | Euplotes focardii |
| 45 | - |
10% of maximum activity | Euplotes focardii |
| 60 | - |
20% of maximum activity | Moneuplotes crassus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
half-life 6.5 min | Moneuplotes crassus |
| 40 | - |
half-life 4.1 min | Euplotes focardii |
| 50 | - |
half-life 1.8 min | Euplotes focardii |
| 50 | - |
half-life 4.2 min | Moneuplotes crassus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 377 | - |
starch | 5°C, pH 9.0 | Moneuplotes crassus | |
| 619 | - |
starch | 25°C, pH 9.0 | Moneuplotes crassus | |
| 718 | - |
starch | 5°C, pH 9.0 | Euplotes focardii | |
| 880 | - |
starch | 35°C, pH 9.0 | Moneuplotes crassus | |
| 982 | - |
starch | 35°C, pH 9.0 | Euplotes focardii | |
| 1466 | - |
starch | 25°C, pH 9.0 | Euplotes focardii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Euplotes focardii |
| 9 | - |
- |
Moneuplotes crassus |
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