Application | Comment | Organism |
---|---|---|
biotechnology | improvement of the thermal stability of alpha-amylase by combinatorial coevolving-site saturation mutagenesis (CCSM), in which the functionally correlated variation sites of proteins are chosen as the hotspot sites to construct focused mutant libraries. Method leads to identification of beneficial mutation sites, and enhances the thermal stability of wild-type alpha-amylase Amy7C by 8°C | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
H100I | increase in half-inactivation temperature, kcat value similar to wild-type | Bacillus subtilis |
H100M/D144R | increase in half-inactivation temperature, 70% decrease in kcat value | Bacillus subtilis |
additional information | improvement of the thermal stability of alpha-amylase by combinatorial coevolving-site saturation mutagenesis (CCSM), in which the functionally correlated variation sites of proteins are chosen as the hotspot sites to construct focused mutant libraries. Method leads to identification of beneficial mutation sites, and enhances the thermal stability of wild-type alpha-amylase Amy7C by 8°C | Bacillus subtilis |
N197C | decrease in half-inactivation temperature, kcat value similar to wild-type | Bacillus subtilis |
T147P | increase in half-inactivation temperature, 25% decrease in kcat value | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | H9B4I9 | - |
- |
Bacillus subtilis CN7 | H9B4I9 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
Amy7C | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58.3 | - |
mutant N197C, half inactivation | Bacillus subtilis |
62.3 | - |
wild-type, half inactivation | Bacillus subtilis |
66.8 | - |
mutant H100I, half inactivation | Bacillus subtilis |
69.3 | - |
mutant T147P, half inactivation | Bacillus subtilis |
70.3 | - |
mutant H100M/D144R, half inactivation | Bacillus subtilis |