Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 25% residual activity | Mycothermus thermophilus | |
Hg2+ | 1 mM, 15% residual activity | Mycothermus thermophilus | |
additional information | not inhibitory: D-glucose | Mycothermus thermophilus | |
Zn2+ | 1 mM, 53% residual activity | Mycothermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Mycothermus thermophilus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by several metal ions tested, including Ca2+ up to 10 mM | Mycothermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
gel filtration | Mycothermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycothermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycothermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.6 | - |
substrate starch, pH 5.5, 60°C | Mycothermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Mycothermus thermophilus | ? | - |
? | |
amylose + H2O | - |
Mycothermus thermophilus | ? | - |
? | |
glycogen + H2O | - |
Mycothermus thermophilus | malto-oligosaccharides | - |
? | |
maltose + H2O | - |
Mycothermus thermophilus | 2 D-glucose | - |
? | |
additional information | substrate preference in decreasing order: starch, amylopectin, maltose, amylose and glycogen | Mycothermus thermophilus | ? | - |
? | |
starch + H2O | - |
Mycothermus thermophilus | maltotriose + maltotetraose + maltopentaose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE | Mycothermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Mycothermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
in absence of substrate stable for 1 h, in presence of substrate fully stable | Mycothermus thermophilus |
60 | - |
half-life 12 min in absence of substrate | Mycothermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Mycothermus thermophilus |