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Literature summary for 3.2.1.1 extracted from

  • Aquino, A.C.; Jorge, J.A.; Terenzi, H.F.; Polizeli, M.L.
    Studies on a thermostable alpha-amylase from the thermophilic fungus Scytalidium thermophilum (2003), Appl. Microbiol. Biotechnol., 61, 323-328 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 25% residual activity Mycothermus thermophilus
Hg2+ 1 mM, 15% residual activity Mycothermus thermophilus
additional information not inhibitory: D-glucose Mycothermus thermophilus
Zn2+ 1 mM, 53% residual activity Mycothermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Mycothermus thermophilus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not activated by several metal ions tested, including Ca2+ up to 10 mM Mycothermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
gel filtration Mycothermus thermophilus

Organism

Organism UniProt Comment Textmining
Mycothermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mycothermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6
-
substrate starch, pH 5.5, 60°C Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Mycothermus thermophilus ?
-
?
amylose + H2O
-
Mycothermus thermophilus ?
-
?
glycogen + H2O
-
Mycothermus thermophilus malto-oligosaccharides
-
?
maltose + H2O
-
Mycothermus thermophilus 2 D-glucose
-
?
additional information substrate preference in decreasing order: starch, amylopectin, maltose, amylose and glycogen Mycothermus thermophilus ?
-
?
starch + H2O
-
Mycothermus thermophilus maltotriose + maltotetraose + maltopentaose
-
?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Mycothermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Mycothermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
in absence of substrate stable for 1 h, in presence of substrate fully stable Mycothermus thermophilus
60
-
half-life 12 min in absence of substrate Mycothermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Mycothermus thermophilus