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Literature summary for 3.2.1.1 extracted from

  • Wu, X.; Wang, Y.; Tong, B.; Chen, X.; Chen, J.
    Purification and biochemical characterization of a thermostable and acid-stable alpha-amylase from Bacillus licheniformis B4-423 (2018), Int. J. Biol. Macromol., 109, 329-337 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Tween 20 8.9% inhibition at 20%, 9.2% activation at 10% Bacillus licheniformis

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 37.6% inhibition at 1 mM, 43.7% at 5 mM Bacillus licheniformis
ascorbate 28.4% inhibition at 5 mM, 34.9% at 10 mM Bacillus licheniformis
Ba2+ 5.3% inhibition at 1 mM, 11.1% at 5 mM Bacillus licheniformis
Co2+ 11.1% inhibition at 1 mM, 89.4% at 5 mM Bacillus licheniformis
Cu2+ complete inhibition at 1-5 mM Bacillus licheniformis
Dimethyl formamide 38.2% inhibition at 1 mM, 72.0% at 5 mM Bacillus licheniformis
DTNB 55.2% inhibition at 5 mM Bacillus licheniformis
DTT 61.9% inhibition at 1 mM, 84.8% at 5 mM Bacillus licheniformis
EDTA 19.3% inhibition at 5 mM Bacillus licheniformis
Fe2+ 94.1% inhibition at 1 mM, complete inhibition at 5 mM Bacillus licheniformis
Fe3+ complete inhibition at 1-5 mM Bacillus licheniformis
H2O2 82.3% inhibition at 0.5%, 43.7% at 5 mM Bacillus licheniformis
Mn2+ 7.1% inhibition at 1 mM, 22.4% at 5 mM Bacillus licheniformis
additional information no effect by 5-10 mM of urea and glycerol, poor effects by 0.5-1% sodium tetraborate Bacillus licheniformis
Pb2+ 74.5% inhibition at 1 mM, complete inhibition at 5 mM Bacillus licheniformis
PMSF 36.5% inhibition at 1mM, 53.7% at 5 mM Bacillus licheniformis
SDS 14.9% inhibition at 2% Bacillus licheniformis
Sodium citrate 23.1% inhibition at 5 mM Bacillus licheniformis
Triton X-100 12.4% inhibition at 10% Bacillus licheniformis
Tween 20 8.9% inhibition at 20%, 9.2% activation at 10% Bacillus licheniformis
Tween 80 8.3% inhibition at 20% Bacillus licheniformis
Zn2+ 59.2% inhibition at 1 mM, 94.3% at 5 mM Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme shows Ca2+-independency, no effect by Ca2+ at 1-5 mM. Poor effects by 1-5 mM of Na+, K+, and Mg2+ Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
about, gel filtration Bacillus licheniformis

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol loss of 83% activity at 10% 2-propanol after 30 min at room temperature Bacillus licheniformis
Acetone loss of 15% activity at 20% acetone after 30 min at room temperature Bacillus licheniformis
Butanol loss of 89% activity at 10% butanol after 30 min at room temperature Bacillus licheniformis
additional information the enzyme activity is poorly affected by 10-20% of methanol, ethanol, chloroform, isoamylalcohol, and hexane after 30 min at room temperature Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
isolated from a hot spring
-
Bacillus licheniformis B4-423
-
isolated from a hot spring
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 8.34fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography Bacillus licheniformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2902.26
-
purified enzyme, pH 5.0, 100°C, substrate starch Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O 1% w/v starch solution Bacillus licheniformis ?
-
?
starch + H2O 1% w/v starch solution Bacillus licheniformis B4-423 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 58000, SDS-PAGE Bacillus licheniformis
More comparison of primary and secondary enzyme structure Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 150 20% of maximal activity at 20°C, 30% at 50°C, 65% at 60°C, 75% at 80°C and 150°C, 90% at 90°C and 110-130°C, profile overview Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 70 purified native enzyme, 10 min, completely stable at Bacillus licheniformis
80
-
purified native enzyme, 10 min, 70% activity remaining Bacillus licheniformis
90
-
purified native enzyme, 10 min, 10% activity remaining Bacillus licheniformis
100
-
purified native enzyme, 10 min, no activity remaining Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
4 10 about 15% of maximal activity at pH 4.0, 75% at pH 8.0, and 70% at pH 10.0, profile overview Bacillus licheniformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 purified native enzyme, highly stable at Bacillus licheniformis