Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Tween 20 | 8.9% inhibition at 20%, 9.2% activation at 10% | Bacillus licheniformis |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 37.6% inhibition at 1 mM, 43.7% at 5 mM | Bacillus licheniformis | |
ascorbate | 28.4% inhibition at 5 mM, 34.9% at 10 mM | Bacillus licheniformis | |
Ba2+ | 5.3% inhibition at 1 mM, 11.1% at 5 mM | Bacillus licheniformis | |
Co2+ | 11.1% inhibition at 1 mM, 89.4% at 5 mM | Bacillus licheniformis | |
Cu2+ | complete inhibition at 1-5 mM | Bacillus licheniformis | |
Dimethyl formamide | 38.2% inhibition at 1 mM, 72.0% at 5 mM | Bacillus licheniformis | |
DTNB | 55.2% inhibition at 5 mM | Bacillus licheniformis | |
DTT | 61.9% inhibition at 1 mM, 84.8% at 5 mM | Bacillus licheniformis | |
EDTA | 19.3% inhibition at 5 mM | Bacillus licheniformis | |
Fe2+ | 94.1% inhibition at 1 mM, complete inhibition at 5 mM | Bacillus licheniformis | |
Fe3+ | complete inhibition at 1-5 mM | Bacillus licheniformis | |
H2O2 | 82.3% inhibition at 0.5%, 43.7% at 5 mM | Bacillus licheniformis | |
Mn2+ | 7.1% inhibition at 1 mM, 22.4% at 5 mM | Bacillus licheniformis | |
additional information | no effect by 5-10 mM of urea and glycerol, poor effects by 0.5-1% sodium tetraborate | Bacillus licheniformis | |
Pb2+ | 74.5% inhibition at 1 mM, complete inhibition at 5 mM | Bacillus licheniformis | |
PMSF | 36.5% inhibition at 1mM, 53.7% at 5 mM | Bacillus licheniformis | |
SDS | 14.9% inhibition at 2% | Bacillus licheniformis | |
Sodium citrate | 23.1% inhibition at 5 mM | Bacillus licheniformis | |
Triton X-100 | 12.4% inhibition at 10% | Bacillus licheniformis | |
Tween 20 | 8.9% inhibition at 20%, 9.2% activation at 10% | Bacillus licheniformis | |
Tween 80 | 8.3% inhibition at 20% | Bacillus licheniformis | |
Zn2+ | 59.2% inhibition at 1 mM, 94.3% at 5 mM | Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme shows Ca2+-independency, no effect by Ca2+ at 1-5 mM. Poor effects by 1-5 mM of Na+, K+, and Mg2+ | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
about, gel filtration | Bacillus licheniformis |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | loss of 83% activity at 10% 2-propanol after 30 min at room temperature | Bacillus licheniformis |
Acetone | loss of 15% activity at 20% acetone after 30 min at room temperature | Bacillus licheniformis |
Butanol | loss of 89% activity at 10% butanol after 30 min at room temperature | Bacillus licheniformis |
additional information | the enzyme activity is poorly affected by 10-20% of methanol, ethanol, chloroform, isoamylalcohol, and hexane after 30 min at room temperature | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
isolated from a hot spring | - |
Bacillus licheniformis B4-423 | - |
isolated from a hot spring | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 8.34fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography | Bacillus licheniformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2902.26 | - |
purified enzyme, pH 5.0, 100°C, substrate starch | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | 1% w/v starch solution | Bacillus licheniformis | ? | - |
? | |
starch + H2O | 1% w/v starch solution | Bacillus licheniformis B4-423 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, SDS-PAGE | Bacillus licheniformis |
More | comparison of primary and secondary enzyme structure | Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Bacillus licheniformis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 150 | 20% of maximal activity at 20°C, 30% at 50°C, 65% at 60°C, 75% at 80°C and 150°C, 90% at 90°C and 110-130°C, profile overview | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | purified native enzyme, 10 min, completely stable at | Bacillus licheniformis |
80 | - |
purified native enzyme, 10 min, 70% activity remaining | Bacillus licheniformis |
90 | - |
purified native enzyme, 10 min, 10% activity remaining | Bacillus licheniformis |
100 | - |
purified native enzyme, 10 min, no activity remaining | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Bacillus licheniformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | about 15% of maximal activity at pH 4.0, 75% at pH 8.0, and 70% at pH 10.0, profile overview | Bacillus licheniformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | purified native enzyme, highly stable at | Bacillus licheniformis |