Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 20% inhibition at 1 mM, 30% at 5 mM | Geobacillus sp. K1C | |
Cu2+ | complete inhibition at 1 mM | Geobacillus sp. K1C | |
Dextran | complete inhibition at 1% | Geobacillus sp. K1C | |
DTT | 20% inhibition at 1 mM | Geobacillus sp. K1C | |
EDTA | over 80% inhibition at 1 mM | Geobacillus sp. K1C | |
ethanol | 20% inhibition at 10%, 40% at 20% | Geobacillus sp. K1C | |
glycerol | 20-25% inhibition at 1-2% | Geobacillus sp. K1C | |
glycine | almost complete inhibition at 1% | Geobacillus sp. K1C | |
Hg2+ | complete inhibition at 1 mM | Geobacillus sp. K1C | |
Isoamylalcohol | 70% inhibition at 10%, 90% at 20% | Geobacillus sp. K1C | |
methanol | 20% inhibition at 10%, 40% at 20% | Geobacillus sp. K1C | |
Mg2+ | 30% inhibition at 1 mM, 50% at 5 mM | Geobacillus sp. K1C | |
Mn2+ | 80% inhibition at 1-5 mM | Geobacillus sp. K1C | |
additional information | no or poor effect by 10-20% of acetone and benzol | Geobacillus sp. K1C | |
Ni2+ | no inhibition at 1 mM, 50% at 5 mM | Geobacillus sp. K1C | |
Pb2+ | 90% inhibition at 1 mM | Geobacillus sp. K1C | |
PEG 8000 | 15-20% inhibition at 1-2% | Geobacillus sp. K1C | |
PMSF | 10% inhibition at 1 mM, 90% at 5 mM | Geobacillus sp. K1C | |
Propanol | 30% inhibition at 10%, 50% at 20% | Geobacillus sp. K1C | |
SDS | 40% inhibition at 0.1-0.2% | Geobacillus sp. K1C | |
Triton X-100 | 15% inhibition at 0.1%, 20% at 0.2% | Geobacillus sp. K1C | |
Tween 20 | 20% inhibition at 0.1-0.2% | Geobacillus sp. K1C | |
Tween 80 | 10% inhibition at 0.1%, 25% at 0.2% | Geobacillus sp. K1C |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the activity of the purified enzyme is Ca2+-independent | Geobacillus sp. K1C |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
about, gel filtration | Geobacillus sp. K1C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. K1C | - |
isolated from Manikaran hot springs, India | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 49fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration | Geobacillus sp. K1C |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26493 | - |
purified native enzyme, pH 6.0, 80°C, substrate soluble starch | Geobacillus sp. K1C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, overview | Geobacillus sp. K1C | ? | - |
? | |
raw corn starch + H2O | - |
Geobacillus sp. K1C | ? | - |
? | |
raw potato starch + H2O | - |
Geobacillus sp. K1C | ? | - |
? | |
raw rice starch + H2O | - |
Geobacillus sp. K1C | ? | - |
? | |
raw wheat starch + H2O | - |
Geobacillus sp. K1C | ? | - |
? | |
soluble starch + H2O | 0.5% starch solution | Geobacillus sp. K1C | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000, SDS-PAGE | Geobacillus sp. K1C |
More | presence of random coil conformation | Geobacillus sp. K1C |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Geobacillus sp. K1C |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 95 | over 50% of maximal activity within this range, profile overview | Geobacillus sp. K1C |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
in the presence of substrate, thermostability of the alpha-amylase is increased | Geobacillus sp. K1C |
70 | - |
purified native enzyme, 40% activity is retained after 2 h, 20% after 5 h in absence of substrate, while in presence of 0.1% starch substrate, over 80% activity is retained for 8 h | Geobacillus sp. K1C |
80 | - |
purified native enzyme, 15% activity is retained after 1 h, 10% after 2 h in absence of substrate, while in presence of 0.1% starch substrate, about 30% activity is retained for 4-8 h, 20% remaining after 9 h | Geobacillus sp. K1C |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Geobacillus sp. K1C |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range, profile overview | Geobacillus sp. K1C |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | purified native enzyme, 90% initial activity is retained after 48 h | Geobacillus sp. K1C |