Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged chimeric acidic alpha-amylase Ba-Gt-amy in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Geobacillus thermoleovorans |
recombinant expression of His-tagged chimeric acidic alpha-amylase Ba-Gt-amy in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Bacillus acidicola |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the chimeric amylase Ba-Gt-amy having catalytic domain from acidic amylase of Bacillus acidicola and N- and C-terminal additional amino acids from thermophilic alpha-amylase of Geobacillus thermoleovorans | Geobacillus thermoleovorans |
additional information | construction of the chimeric amylase Ba-Gt-amy having catalytic domain from acidic amylase of Bacillus acidicola and N- and C-terminal additional amino acids from thermophilic alpha-amylase of Geobacillus thermoleovorans | Bacillus acidicola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Bacillus acidicola | |
Ag+ | - |
Geobacillus thermoleovorans | |
Cu2+ | - |
Bacillus acidicola | |
Cu2+ | - |
Geobacillus thermoleovorans | |
Hg2+ | strong inhibition | Bacillus acidicola | |
Hg2+ | strong inhibition | Geobacillus thermoleovorans | |
Mn2+ | - |
Bacillus acidicola | |
Mn2+ | - |
Geobacillus thermoleovorans | |
Ni2+ | - |
Bacillus acidicola | |
Ni2+ | - |
Geobacillus thermoleovorans | |
Pb2+ | - |
Bacillus acidicola | |
Pb2+ | - |
Geobacillus thermoleovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Lineweaver-Burk plot, kinetics and thermodynamics for recombinant chimeric acidic alpha-amylase Ba-Gt-amy, detailed overview. Adsorption kinetics of mutant Ba-Gt-amy and wild-type Ba?amy to 1% raw corn starch. Km and Vmax values of mutant Ba-Gt-amy for soluble starch are 0.8 mg/ml and 10.746 mmol*mg/min, whereas those for wild-type Ba-amy are 1.66 mg/ml and 0.0526 mmol*mg/min, respectively | Bacillus acidicola | |
additional information | - |
additional information | Lineweaver-Burk plot, kinetics and thermodynamics for recombinant chimeric acidic alpha-amylase Ba-Gt-amy, detailed overview. Adsorption kinetics of mutant Ba-Gt-amy to 1% raw corn starch. Km and Vmax values of mutant Ba-Gt-amy for soluble starch are 0.8 mg/ml and 10.746 mmol * mg/min | Geobacillus thermoleovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates the recombinant chimeric mutant amylase | Geobacillus thermoleovorans | |
Co2+ | activates the recombinant chimeric mutant amylase | Bacillus acidicola | |
Mg2+ | activates the recombinant chimeric mutant amylase | Geobacillus thermoleovorans | |
Mg2+ | activates the recombinant chimeric mutant amylase | Bacillus acidicola | |
additional information | the recombinant chimeric mutant amylase activity is not significantly affected by EGTA, Ca2+, Na+, and K+. The enzymes are Ca2+-independent | Geobacillus thermoleovorans | |
additional information | the recombinant chimeric mutant amylase activity is not significantly affected by EGTA, Ca2+, Na+, and K+. The enzymes are Ca2+-independent | Bacillus acidicola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus acidicola | - |
- |
- |
Geobacillus thermoleovorans | J9PXA2 | - |
- |
Geobacillus thermoleovorans MTCC 4220 | J9PXA2 | - |
- |
Geobacillus thermoleovorans NP-54 | J9PXA2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged chimeric acidic alpha-amylase Ba-Gt-amy from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Geobacillus thermoleovorans |
recombinant His-tagged chimeric acidic alpha-amylase Ba-Gt-amy from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bacillus acidicola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
corn starch + H2O | 1% starch solution | Geobacillus thermoleovorans | maltose + maltotriose + maltotetraose | - |
? | |
corn starch + H2O | 1% starch solution | Bacillus acidicola | maltose + maltotriose + maltotetraose | - |
? | |
corn starch + H2O | 1% starch solution | Geobacillus thermoleovorans NP-54 | maltose + maltotriose + maltotetraose | - |
? | |
corn starch + H2O | 1% starch solution | Geobacillus thermoleovorans MTCC 4220 | maltose + maltotriose + maltotetraose | - |
? | |
additional information | applicability of recombinant chimeric mutant Ba-Gt-amy and wild-type amylase Ba-amy in raw starch hydrolysis, method evaluation | Bacillus acidicola | ? | - |
? | |
additional information | applicability of recombinant chimeric mutant Ba-Gt-amy in raw starch hydrolysis, method evaluation | Geobacillus thermoleovorans | ? | - |
? | |
additional information | applicability of recombinant chimeric mutant Ba-Gt-amy in raw starch hydrolysis, method evaluation | Geobacillus thermoleovorans NP-54 | ? | - |
? | |
additional information | applicability of recombinant chimeric mutant Ba-Gt-amy in raw starch hydrolysis, method evaluation | Geobacillus thermoleovorans MTCC 4220 | ? | - |
? | |
potato starch + H2O | 0.5% starch solution | Geobacillus thermoleovorans | maltose + maltotriose + maltotetraose | - |
? | |
potato starch + H2O | 0.5% starch solution | Bacillus acidicola | maltose + maltotriose + maltotetraose | - |
? | |
potato starch + H2O | 0.5% starch solution | Geobacillus thermoleovorans NP-54 | maltose + maltotriose + maltotetraose | - |
? | |
potato starch + H2O | 0.5% starch solution | Geobacillus thermoleovorans MTCC 4220 | maltose + maltotriose + maltotetraose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acidic amylase | - |
Bacillus acidicola |
Ba-amy | - |
Bacillus acidicola |
Gt-amy | - |
Geobacillus thermoleovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant chimeric acidic alpha-amylase Ba-Gt-amy | Geobacillus thermoleovorans |
60 | - |
recombinant wild-type Ba-amy enzyme | Bacillus acidicola |
60 | 70 | recombinant chimeric mutant Ba-Gt-amy | Bacillus acidicola |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
determination of thermal denaturation kinetics of the recombinant His-tagged chimeric acidic alpha-amylase Ba-Gt-amy, overview | Geobacillus thermoleovorans |
additional information | - |
determination of thermal denaturation kinetics of the recombinant His-tagged chimeric acidic alpha-amylase Ba-Gt-amy, overview | Bacillus acidicola |
70 | - |
half-life of purified recombinant chimeric mutant Ba-Gt-amy is 44 min, of recombinant wild-type Ba-amy 25 min | Bacillus acidicola |
70 | - |
half-life of purified recombinant chimeric mutant Ba-Gt-amy is 44 min, of recombinant wild-type Gt-amy 238 min | Geobacillus thermoleovorans |
80 | - |
half-life of purified recombinant chimeric mutant Ba-Gt-amy is 30 min | Geobacillus thermoleovorans |
80 | - |
half-life of purified recombinant chimeric mutant Ba-Gt-amy is 30 min, of recombinant wild-type Ba-amy 15 min | Bacillus acidicola |
90 | - |
half-life of purified recombinant chimeric mutant Ba-Gt-amy is 15 min | Geobacillus thermoleovorans |
90 | - |
half-life of purified recombinant chimeric mutant Ba-Gt-amy is 15 min, of recombinant wild-type Ba-amy 5 min | Bacillus acidicola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | recombinant chimeric acidic alpha-amylase Ba-Gt-amy, assay at | Geobacillus thermoleovorans |
4 | 5 | recombinant wild-type Ba-amy enzyme and chimeric mutant Ba-Gt-amy | Bacillus acidicola |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | recombinant chimeric acidic alpha-amylase Ba-Gt-amy, activity range | Geobacillus thermoleovorans |
3 | 6 | recombinant chimeric acidic alpha-amylase Ba-Gt-amy, activity range | Bacillus acidicola |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | purified recombinant chimeric acidic alpha-amylase Ba-Gt-amy, 12 h, 90% activity remaining | Geobacillus thermoleovorans |
4 | 5 | purified recombinant chimeric acidic alpha-amylase Ba-Gt-amy, 12 h, 90% activity remaining | Bacillus acidicola |