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Literature summary for 3.2.1.1 extracted from

  • Xu, Q.; Cao, Y.; Li, X.; Liu, L.; Qin, S.; Wang, Y.; Cao, Y.; Xu, H.; Qiao, D.
    Purification and characterization of a novel intracellular alpha-amylase with a wide variety of substrates hydrolysis and transglycosylation activity from Paenibacillus sp. SSG-1 (2018), Protein Expr. Purif., 144, 62-70 .
    View publication on PubMed
Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Paenibacillus sp. SSG-1

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 85.9% inhibition at 1 mM Paenibacillus sp. SSG-1
Co2+ 75.9% inhibition at 1 mM Paenibacillus sp. SSG-1
Cu2+ 64.1% inhibition at 1 mM Paenibacillus sp. SSG-1
DTT 20.1% inhibition at 1 mM Paenibacillus sp. SSG-1
EDTA 81.9% inhibition at 1 mM Paenibacillus sp. SSG-1
Fe2+ 55.3% inhibition at 1 mM Paenibacillus sp. SSG-1
Li+ 26.2% inhibition at 1 mM Paenibacillus sp. SSG-1
Mn2+ 49.1% inhibition at 1 mM Paenibacillus sp. SSG-1
Ni2+ 65.9% inhibition at 1 mM Paenibacillus sp. SSG-1
Pb2+ 80.6% inhibition at 1 mM Paenibacillus sp. SSG-1
PMSF 21.2% inhibition at 1 mM Paenibacillus sp. SSG-1
SDS 71.0% inhibition at 1 mM Paenibacillus sp. SSG-1
Urea 58.5% inhibition at 1 mM Paenibacillus sp. SSG-1
Zn2+ 80.5% inhibition at 1 mM Paenibacillus sp. SSG-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 soluble starch pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
1.42
-
 maltoheptaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
1.92
-
 maltopentaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
2.28
-
 maltotriose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
2.86
-
 maltose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.25
-
 amylose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Paenibacillus sp. SSG-1 5737
-
intracellular
-
 Paenibacillus sp. SSG-1 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates 7.4% at 1 mM Paenibacillus sp. SSG-1
Ca2+ activates 28.2% at 1 mM Paenibacillus sp. SSG-1
Mg2+ activates 6.9% at 1 mM Paenibacillus sp. SSG-1
additional information poor effects by 1 mM of K+ and Na+ Paenibacillus sp. SSG-1

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. SSG-1 A0A0U4VEK2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Paenibacillus sp. SSG-1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
122.04
-
 purified recombinant enzyme, pH 6.5, 40°C, substrate amylose Paenibacillus sp. SSG-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 71.1% activity compared to amylose Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
amylose + H2O best substrate Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
beta-cyclodextrin + H2O 39.0% activity compared to amylose Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
glycogen + H2O 40.2% activity compared to amylose Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
maltoheptaose + H2O
-
 Paenibacillus sp. SSG-1 D-glucose + maltose + ?
-
 ?
maltopentaose + H2O
-
 Paenibacillus sp. SSG-1 D-glucose + maltose + ?
-
 ?
maltose + H2O
-
 Paenibacillus sp. SSG-1 2 D-glucose
-
 ?
maltotriose + H2O
-
 Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
additional information the enzyme Amy can hydrolyze typical substrates into glucose and maltose, substrate specificity, overview. Enzyme Amy broad substrate range and transglycosylation activity when compared with intracellular alpha-amylase AmyB. Amy exhibits transglycosylation activity when reacted with maltose and maltotriose Paenibacillus sp. SSG-1 ?
-
 ?
pullulan + H2O 54.4% activity compared to amylose Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
soluble starch + H2O 93.3% activity compared to amylose Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?

Subunits

Subunits Comment Organism
? x * 71700, recombinant His-tagged enzyme, SDS-PAGE Paenibacillus sp. SSG-1

Synonyms

Synonyms Comment Organism
Amy
-
 Paenibacillus sp. SSG-1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 45 recombinant His-tagged enzyme Paenibacillus sp. SSG-1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 55 activity range, profile overview Paenibacillus sp. SSG-1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 40 purified recombinant His-tagged enzyme, over 80% activity remaining Paenibacillus sp. SSG-1
50
-
 purified recombinant His-tagged enzyme, 60% activity remaining Paenibacillus sp. SSG-1
60
-
 purified recombinant His-tagged enzyme, 10% activity remaining Paenibacillus sp. SSG-1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.72
-
 soluble starch pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
9.21
-
 amylose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
428.98
-
 maltose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
538.95
-
 maltotriose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
567.01
-
 maltopentaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
573.8
-
 maltoheptaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 recombinant His-tagged enzyme Paenibacillus sp. SSG-1

pH Range

pH Minimum pH Maximum Comment Organism
5.6 8 50% of maximal activity of recombinant His-tagged enzyme within this range, inactive below pH 4.5 Paenibacillus sp. SSG-1

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 purified recombinant His-tagged enzyme, 30% activity remaining Paenibacillus sp. SSG-1
5.5 7.5 purified recombinant His-tagged enzyme, 80% activity remaining Paenibacillus sp. SSG-1
9
-
 purified recombinant His-tagged enzyme, 30% activity remaining Paenibacillus sp. SSG-1

General Information

General Information Comment Organism
evolution enzyme Amy from Paenibacillus sp. SSG-1 exhibits unexpectedly sequence similarity and evolutionary relationships with alpha-glucanotransferase, homology modelling, using the crystal structure of a secretory alpha-amylase AmyA (PDB ID: 1WZA) as the template, and phylogenetic analysis Paenibacillus sp. SSG-1
additional information docking study of the enzyme with maltose Paenibacillus sp. SSG-1
physiological function enzyme Amy may play an important role in utilizing maltooligosaccharides that are released from extracellular alpha-glucan or storage alpha-glucan (glycogen) in Paenibacillus sp. SSG-1 Paenibacillus sp. SSG-1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.83
-
 amylose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
5.41
-
 soluble starch pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
150.17
-
 maltose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
236.38
-
 maltotriose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
295.32
-
 maltopentaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
405.03
-
 maltoheptaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1