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Literature summary for 3.2.1.103 extracted from
- Isolation and characterization of an endo-beta-galactosidase from a new strain of Escherichia freundii (1980), J. Biol. Chem., 255, 5955-5959.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | useful for studying the structure of glycoproteins and oligosaccharides | Citrobacter freundii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, complete inhibition | Citrobacter freundii |  |
| Cu2+ | 1 mM, 30% inhibition | Citrobacter freundii | |
| Hg2+ | 1 mM, complete inhibition | Citrobacter freundii | |
| PCMB | 0.25 mM, complete inhibition | Citrobacter freundii | |
| Zn2+ | 1 mM, 30% inhibition | Citrobacter freundii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
gel filtration | Citrobacter freundii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Citrobacter freundii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Citrobacter freundii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Citrobacter freundii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, stable for several months | Citrobacter freundii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O | - |
Citrobacter freundii | Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Gal + Glc-Cer | - |
? | |
| Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O | - |
Citrobacter freundii | ? | - |
? | |
| Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O | - |
Citrobacter freundii | Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Gal + Glc-Cer | - |
? | |
| Galbeta1-4GlcNAcbeta1-3Galbeta1-3Galbeta1-4Glc-Cer + H2O | - |
Citrobacter freundii | Galbeta1-4GlcNAcbeta1-3Galbeta1-3Gal + Glc-Cer | - |
? | |
| keratan sulfate + H2O | - |
Citrobacter freundii | keratan oligosaccharides | 6-O-sulfo-GlcNAcbeta1-3Gal is the major product | ? | |
| NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O | - |
Citrobacter freundii | NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Gal + Glc-Cer | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
1 h, 50% loss of activity, bovine serum albumin, 0.4 mg/ml, prevents inactivation | Citrobacter freundii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 5.8 | hydrolysis of keratan sulfate from nasal cartilage or Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer | Citrobacter freundii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
17 h at room temperature, rapid loss of activity below | Citrobacter freundii |
| 4.5 | 5.5 | 17 h at room temperature, retains 80 to 100% activity | Citrobacter freundii |
| 6 | - |
17 h at room temperature, rapid loss of activity above | Citrobacter freundii |
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