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Literature summary for 3.2.1.123 extracted from

  • Horibata, Y.; Okino, N.; Ichinose, S.; Omori, A.; Ito, M.
    Purification, characterization, and cDNA cloning of a novel acidic endoglycoceramidase from the jellyfish, Cyanea nozakii (2000), J. Biol. Chem., 275, 31297-31304.
    View publication on PubMed
Search for more literature for 3.2.1.123

Activating Compound

Activating Compound Comment Organism Structure
detergent required Cyanea nozakii
Lubrol Px optimal concentration: 0.4% Cyanea nozakii
Triton X-100 activates at 0.2% Cyanea nozakii

Cloned(Commentary)

Cloned (Comment) Organism
EGCase and EGCase P Cyanea nozakii

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ complete inhibition at 2 mM Cyanea nozakii
SDS complete inhibition at 0.1% Cyanea nozakii
taurodeoxycholate strong inhibitor Cyanea nozakii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide pH 3, 37°C Cyanea nozakii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 gel filtration Cyanea nozakii

Organism

Organism UniProt Comment Textmining
Cyanea nozakii Q9GV16 jellyfish
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme contains 6 potential N-glycosylation sites Cyanea nozakii

Purification (Commentary)

Purification (Comment) Organism
EGCase and EGCase P Cyanea nozakii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.52
-
-
 Cyanea nozakii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Cyanea nozakii Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GD1b Cyanea nozakii Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O weak substrate Cyanea nozakii Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. asialo-GM1 Cyanea nozakii Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-4)Glcbeta(1-1)ceramide + H2O weak substrate Cyanea nozakii Galbeta(1-4)Glc + ceramide
-
 ?
Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. lactosylceramide Cyanea nozakii Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GT1b Cyanea nozakii NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM3 Cyanea nozakii NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GQ1b Cyanea nozakii NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GD3 Cyanea nozakii NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE Cyanea nozakii
monomer 1 * 51000, SDS-PAGE Cyanea nozakii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Cyanea nozakii

pI Value

Organism Comment pI Value Maximum pI Value
Cyanea nozakii calculated from amino acid sequence
-
 5.61